罗格列酮对大鼠腹膜透析相关性腹膜纤维化的影响

目的观察罗格列酮(rosiglitazone,RGZ)对腹膜透析所致腹膜纤维化的影响。方法 50只SD大鼠随机分为6组:空白对照组(n=5)、生理盐水(NS)组、模型组、二甲亚砜(DMSO)组、低浓度RGZ组、高浓度RGZ组(后5组n=9)。除空白对照组外其余大鼠均置入腹透管,除空白组和NS组外其余组均采用高糖腹膜透析液+红霉素制作慢性腹膜透析无菌性腹膜纤维化的大鼠模型。DMSO组、低浓度RGZ组、高浓度RGZ组分别采用DMSO、1.5mg/kg RGZ、15mg/kg RGZ治疗。透析5周后行1h腹膜平衡试验,检测血糖及血脂,计算超滤量(UF)、初始腹透液与透出液葡萄糖比值(D1/D0)、透出液与血浆尿素氮比值(D/Purea);处死大鼠,取壁层腹膜行H-E及Masson染色,观察腹膜形态改变,半定量计算腹膜单位面积血管数及炎症细胞数;免疫组化检测腹膜转化生长因子(TGF)-β1和α-平滑肌肌动蛋白(α-SMA)的表达情况。结果与空白对照组相比,模型组和DMSO组间皮下基质增厚,血管增生及炎症细胞浸润增加(P<0.05);TGF-β1、α-SMA表达水平增高(P<0.05);RGZ能改善上述病理改变,下调TGF-β1、α-SMA表达(P<0.05);与空白对照组比较,NS组、模型组、DMSO组、低浓度RGZ组及高浓度RGZ组的超滤量和D1/D0减少,D/Purea增加(P<0.05),其中以DMSO组及模型组改变最为显著。两种剂量RGZ间差异无统计学意义(P>0.05)。结论在大鼠腹膜透析模型中加用罗格列酮能有效保护腹膜功能,抑制腹膜纤维化。

Influence of rosiglitazone on peritoneal fibrosis induced by peritoneal dialysate in rats

Objective To investigate the effect of rosiglitazone(RGZ) in prevention of peritoneal fibrosis induced by peritoneal dialysis in rats.Methods Fifty SD rats were randomly divided into six groups: control group(n=5),normal saline(NS) group(n=9),model group(n=9),dimethyl sulfoxide(DMSO) group(n=9),low-dose RGZ group(n=9),and high-dose RGZ group(n=9).Peritoneal catheters were implanted in the last five groups,and peritoneal fibrosis models were induced in the last four groups by high-glucose peritoneal dialysat...