脑缺血再灌注后IL-8与微血管炎症损伤关系的实验研究

目的 探讨白细胞介素-8(IL-8)在大鼠全脑缺血再灌注(ischemia reperfusion，IR)血管内皮细胞损伤过程中的作用。方法 将大鼠随机分为假手术组和脑缺血30min再灌注1、3、6、12、24、48、72 h组，动物模型采用全脑缺血模型三血管阻塞法，用免疫组化法检测脑组织IR不同时间点层粘连蛋白表达以标记锻血管损伤与修复过程，同时用原位杂交方法检测IL-8mRNA的表达变化。结果 IR后1hIL-8mRNA表达出现上调，24h达高峰。层粘连蛋白在假手术组呈强阳性表达，IR后3h表达下降，24～48h表达最低，72h表达开始恢复。结论 IR微血管炎症损伤主要发生在早期，此过程中IL-8mRNA表达上调，说明IL-8参与了脑缺血后微血管炎症损伤过程。

Experimental Study of IL-8 and Microvascular Inflammatory Damage after Cerebral Ischemia-reperfusion

Objective To study the role of P-selectin in microvascular inflammtory damage after cerebral ischemia -reperfusion. Methods The rats were randomly divided into the groups as sham operated control group and ischemia-reperfusion 1 h, 3 h, 6 h, 12 h,24 h, 48 h, 72 h groups. The global cerebral ischemia-reperfusion model was formed by occluding three arteries. The expression of laminin was evaluated by immunohistochemical methold, and IL-8 mRNA was detected by in situ hybridization. Results The expression of IL-8 mRNA was increased as early as 1 h after reperfusion and peaked at 24 h after reperfusion. At 72 h after reperfusion, it was still higher than that of sham operated control group. The expression of laminin was strong positive in sham operated control group.At 3 h after reperfusion, the expression of laminin began to decrease, and its lowest level was during 24-48 h. At 72 h, the expression began to reappear. Conclusions The microvascular inflammatory damage appeared in early period after cerebral ischemia-reperfusion. IL-8 was involved in the inflammatory damage of endothelial cell during global cerebral ischmia-reperfusion.