平阳霉素局部注射对鼻息肉病息肉组织中嗜酸性粒细胞凋亡的影响

目的 :通过观察平阳霉素治疗前后鼻息肉病鼻息肉组织中嗜酸性粒细胞凋亡情况 ,探讨平阳霉素治疗鼻息肉病的机制。方法 :平阳霉素 4mg息肉内注射 ,每周 1次 ,5次为 1个疗程 ;应用末端脱氧核苷酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法 (TUNEL法 )检测凋亡细胞 ,与相邻连续的苏木精 伊红染色片对位对照观察嗜酸性粒细胞的凋亡情况。结果 :治愈 7例 ,好转 12例 ,治愈率为 36 .8% ,好转率为 6 3.2 % ;平阳霉素治疗前鼻息肉病组嗜酸性粒细胞表达 (35 .0 3± 19.90 )个 ]显著高于对照组 (5 .2 1± 6 .4 3)个 ](P <0 .0 1) ;治疗后鼻息肉病组嗜酸性粒细胞表达 (7.6 4± 1.4 2 )个 ]明显低于治疗前 (P <0 .0 1) ;治疗后鼻息肉病组嗜酸性粒细胞AI(4 4 .4 7± 8.97) % ]高于治疗前 (2 0 .5 3± 7.6 6 ) % ](P <0 .0 1)。结论 :嗜酸性粒细胞是鼻息肉病鼻息肉组织中主要的浸润炎性细胞 ;平阳霉素可通过促进嗜酸性粒细胞凋亡达到治疗鼻息肉病的目的。

Eosinophil apoptosis in nasal polyposis tissue after bleomycin A5 local injection

Objective:To study the expression of eosinophil in nasal polyposis tissue after Bleomycin A 5 local injection.Method:Nineteen cases nasal polyposis specimen obtained before and after treatment with Bleomycin A 5 were studied with terminal deoxynucleotidyl transferasemediated dUTP nick endlabeling(TUNEL).Result:The number of eosinophil in nasal polyposis specimen before treatment is significantly higher than that after treatment (P<0.01) and so to normal inferior turbinate concha mucosa (P<0.01). The apoptosis index(AI) of eosinophil after treatment is significantly higher than that before treatment (P<0.01).Conclusion:Eosinophil is the main infiltration cell in nasal polyposis tissue. Bleomycin A 5 could cure nasal polyposis by promoting eosinophil apoptosis.