罗格列酮对大鼠骨密度和骨微结构的影响

目的研究罗格列酮对去势和不去势大鼠骨密度和骨微结构的影响。方法取32只6月龄SD雌性大鼠随机分为两组,双侧卵巢摘除或仅切除双侧卵巢周围少量脂肪组织,术后三周将全部大鼠随机分为四组,每组8只。sham（R）组和OVX（R）组以罗格列酮每日2 mg/kg溶解于1 m1生理盐水灌胃,Sham（NS）组和OVX（NS）以生理盐水每日1 ml灌胃8周,观察大鼠股骨和腰椎骨密度、骨微结构等指标。结果去势组腰椎骨密度明显降低,OVX（NS）组和OVX（R）组较sham（NS）组分别降低8.5%和14%,OVX（R）组较sham（R）组降低9.1%。OVx（R）组股骨和腰椎骨小梁骨量较sham（NS）组分别减少41.3%和87.5%,较sham（R）组减少37.9%和78.9%。OVX（R）组股骨和腰椎骨小梁厚度较sham（NS）组分别减少11.8%和41.4%,较sham（R）组减少11.8%和33.3%。结论罗格列酮可使大鼠骨密度降低,骨小梁骨量减少,在雌激素缺乏时,罗格列酮对大鼠骨骼破坏加倍,骨密度显著降低,骨微结构明显受损。

Effect of rosiglitazone on bone mineral density and bone microstructure in rats

Objective To investigate the effects of rosiglitazone on bone mineral density and bone microstructure in rats.Methods Thirty two SD female rats aged 6 months were ovariectomized or sham operated.After three weeks,they are randomly assigned into four groups（n = 8 in each）：the sham（R） group and the OVX（R） group received intragastric administration of rosiglitazone 2 mg for 8 weeks,and the sham（NS） group and the OVX（NS） group received intragastric administration of normal saline（1 ml ？ day~（-1）） for 8 weeks.Bone mineral density（BMD） of femur and lumbar were determined by dual-energy X-ray absorptiometry（DXA）.Bone microstructure including trabecular bone volume and trabecular thickness were measured by micro-CT.Results Compared with sham （NS） group,the BMD of lumbar of OVX（NS） group and the OVX（R） group declined by 8.5%and 14%,respectively.The OVX（R） group declined by 9.1%compared with group sham（R）. Compared with the sham（NS） group,the trabecular volume of femur and lumbar in OVX（R） group decreased by 41.3%and 87.5%;while the OVX（R） group decreased by 37.9%and 78.9% compared with the sham（R） group.In addition,the trabecular thickness of femur and lumbar of rats of the OVX（R） group is lower than the sham（NS） group,by 11.8%and 41.4%,while they declined by 11.8%and 33.3%compared with the sham（R） group.Conclusion Rosiglitazone may decrease the BMD and trabecular volume of rats,particularly for the ovariectomized rats.