Antisera Recognising HLA-A, -B and DRW Antigens Raised in Rhesus Monkeys |
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| Authors: | C J Barnstable D Snary M J Crumpton H Balner W F Bodmer |
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| Institution: | Genetics Laboratory, Dept. of Biochemistry, Oxford;National Institute for Medical Research, Mill Hill London, England;Primate Centre, TNO, Rijswijk (ZH), The Netherlands;(Present Address) Department of Neurobiology, Harvard Medical School, Boston, Massachusetts, USA;5 (Present Address) Department Immunochemistry, Wellcome Research Laboratories, Langley Court, Beckenham, Kent;(Present address) Imperial Cancer Research Fund, Lincoln's Inn Fields, London, England |
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| Abstract: | Rhesus monkeys were immunized with partially purified HLA-A, -B, -C and DR antigens. The resulting sera were shown to have activity against species-specific determinants on both HLA-A, -B, -C chains and beta 2 microglobulin by the use of somatic cell hybrids. When this was removed by absorption, the sera showed activity against three of the four HLA-A and -B antigens in the immunogen when tested on a panel of peripheral blood lymphocytes and T cells. Antibodies recognizing HLA-DR antigens were detected by testing platelet absorbed sera on a panel of typed lymphoblastoid cell lines. After absorption to remove activity against species-specific determinants on the HLA-DR antigens, two cross reacting specificities were defined. One consisted of a determinant in common between HLA-DRw1, 2 and 6 and the other a putative determinant in common between HLA-DRw4, and 5. The nature and significance of these cross-reacting groups of HLA-DR antigens is discussed in the light of current HLA-DR serology and the nature of HLA antigens in general. |
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