Measurement of 5-aminolaevulinic acid by reversed phase HPLC and fluorescence detection |
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| Authors: | E I Minder |
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| Institution: | 1. Department of Pharmaceutical, Chemical and Environmental Sciences, Faculty of Engineering and Science, University of Greenwich at Medway, Central Avenue, Chatham Maritime, Kent ME4 4TB, United Kingdom;2. School of Pharmacy, Faculty of Science and Engineering, University of Wolverhampton, Wolverhampton, West Midlands WV1 1LY, United Kingdom;3. Department of Pharmacy, University of Huddersfield, Queensgate, Huddersfield HD1 3DH, United Kingdom;4. Research and Development Department, Bristol Laboratories Limited, Laporte Way, Luton, Bedfordshire LU4 8WL, United Kingdom;1. Centre de Physique Atomique Moléculaire et d’Optique Quantique (CEPAMOQ), Faculty of Science, University of Douala, P.O. Box 8580, Douala, Cameroon;2. Laboratoire de Spectroscopie Atomique Moléculaire et Applications (LSAMA), Faculty of Science, University of Tunis El Manar, P.O. Box 2092, Tunis, Tunisia;3. University of Maroua, P.O. Box 46, Maroua, Cameroon;1. Center for Proteomics and Metabolomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquatic Food Safety, School of Life Sciences, Sun Yat-sen University, University City, Guangzhou 510006, PR China;2. Department of Biological Sciences, The University of Texas, El Paso, TX 79968, USA;2. Department of Chemistry, University of Minho, Gualtar, 4710-057 Braga, Portugal;1. YOuth in COnservation of CUltural Heritage, YOCOCU, Largo dei Quintili, 21, 00175 Rome, Italy;2. Dept. of Information Engineering, Electronics and Telecommunications, “La Sapienza”, University of Rome, Via Eudossiana 18, 00184 Rome, Italy;3. Dept. of Biology, Ecology and Earth Science, University of Calabria, Via Pietro Bucci, 87036, Arcavacata di Rende, CS, Italy |
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| Abstract: | A new method for sensitive measurement of delta-aminolaevulinic acid (ALA) in biological material is described. ALA is derivatized with dansyl chloride, separated by HPLC and estimated using a fluorescence detector. The pretreatment of biological samples includes desamination of L-alpha-aminoacids with L-aminoacid-oxidase before dansylation. The sensitivity of the method is slightly below 1 pmol/injection for standards and the lower limit of quantification is 0.1 mumol/l for plasma and 10 nmol/l for cerebrospinal fluid. Reference values in plasma are 3.53 +/- 1.75 (SD) (n = 43) mumol/l and in packed erythrocytes they ranged from 6 to 26 mumol/l (mean: 14.0 +/- 5.5 mumol/l). In cerebrospinal fluid of non-porphyric individuals less than 2 nmol/l were recovered. |
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