阿魏酸钠对氧化损伤大鼠晶状体上皮细胞凋亡的防护

目的　探讨阿魏酸钠 (SF)对实验性氧化损伤大鼠晶状体上皮细胞 (LEC)凋亡有无抑制作用。方法　采用无菌操作摘取SD大鼠双眼 ,并在手术显微镜下分离晶状体 ,随机分成空白对照组、过氧化氢组 (H2 O2 )、吡诺克辛 (PS)组和SF组。使晶状体孵育在 30 0 μmol·L- 1H2 O2 培养液中复制LEC凋亡模型 ,同时加入终浓度为 5mmol·L- 1的SF ,置CO2 培养箱共同孵育 2 4h。取晶状体前囊膜 ,采用TUNEL法检测LEC凋亡及凋亡率、透射电子显微镜观察LEC超微结构改变和凋亡小体形成。结果　H2 O2组LEC凋亡率 (92 .0± 2 .6 ) %显著高于空白对照组(3.5± 1.8) %;SF组LEC凋亡率 (2 0 .8± 3.0 ) %显著低于H2 O2 组 ,且与PS组 (5 6 .0± 9.9) %有非常显著的差异。超微结构研究显示 ,H2 O2 组绝大多数LEC发生凋亡 ,并呈凋亡各期严重改变的全过程 ;SF组仅少数LEC发生凋亡 ,并多为早期或中期的轻微改变。结论　SF可明显抑制实验性氧化损伤大鼠LEC凋亡 ,且明显优于PS滴眼液。

Sodium ferulate protecting against apoptosis of lens epithelial cell injured by oxidation

AIM To investigate if sodium ferulate inhibits the apoptosis of lens epithelial cell (LEC) resulted from the experimental oxidative injury. METHODS Eyes in SD rats were excised and lenses were separated under operating microscope and sterilized condition. Lenses were divided randomly into four groups: control group, hydrogen peroxide group (H₂O₂), pirenoxine sodium group (PS) and sodium ferulate group (SF). Lenses were incubated in CO₂ incubator for 24 h with 300 μmol·L^-1 H₂O₂ and with or without 5.3 mg·L^-1 PS or 5 mmol·L^-1 SF. LEC apoptosis and apoptosis rate were measured by terminal deoxynucle otidyl transferase mediated biotin-dUTP nick end labeling (TUNEL) method. Ultrastructure changes and apoptosis bodies of LEC were observed under transmission electron microscope. RESULTS The results showed: ① Apoptosis rate in H₂O₂ group (92.0±2.6)% was significantly higher than that in control group (3.5±1.8)%. Apoptosis rate in SF group (20.8±3.0)% was remarkably lower than that in H₂O₂ group and PS group. ② Ultrastructure observation indicated that apoptosis cells occurred in most LEC of H₂O₂ group and the changes were severe and presenting different stages. While a few apoptosis cells were observed in SF group, the changes were slight and most of them were in early and middle stages. CONCLUSION ]The study indicated that SF inhibited apoptosis of LEC of experimental oxidative injury significantly, the effects were stronger than PS.