CD15及CD133在高、低级别胶质瘤中的表达及意义

目的探讨CD15在胶质瘤的表达及分布特征，与目前公认的脑肿瘤干细胞标志物CD133作对照，并探讨两者相关性。方法采用免疫组织化学法检测80例胶质瘤组织（高级别39例，低级别41例）CD15和CD133的表达。用Image-Pro Plus 6.0图像分析处理软件计算CD15和CD133阳性细胞积分光密度值（IOD）。结果CD15与CD133在高、低级别的胶质瘤均有表达。CD15及CD133阳性细胞数以及壁龛随胶质瘤病理级别增高而增多。在高、低级别胶质瘤中CD15⁺细胞与CD133⁺细胞IOD值差异均有统计学意义（z=－3.432，P=0.001；z=－4.192，P=0.000）；CD15⁺细胞与CD133⁺细胞IOD值呈显著正相关（r=0.535，P=0.000）；CD133还表达于含有肥胖细胞成分的胶质瘤肥胖细胞中，并可见CD133+血管。结论CD15及CD133阳性细胞数以及壁龛随胶质瘤病理级别增高而增多。CD15及CD133表达成正相关关系。含有肥胖细胞成分的胶质瘤中大量肥胖细胞CD133阳性，与其不良临床预后相符。关键词：

Expression and Significance of CD15 and CD133 in High-and Low-grade Gliomas

ObjectiveTo investigate the expression and distribution characteristics of CD15 and CD133 in gliomas, and analyze the correlation between CD15 and CD133.MethodsImmunohistochemistry stainning was used to detect the expression of CD15 and CD133 in gliomas. The integrated optical density(IOD) of CD15+ cells and CD133⁺ cells in different grades of gliomas was calculated by Image-Pro Plus 6.0 Image analysis software.ResultsAlong with the tumor grade increased,the number of CD15⁺ and CD133⁺ cells and their niches was increased significantly.The IOD was significant difference between CD15⁺ cells and CD133⁺ cells in high-or low-grade gliomas（z=-3.432，P=0.001；z=-4.192，P=0.000）; The IOD of CD15⁺ cells was positive correlation to CD133⁺ cells (r=0.535, P=0.000). CD133 also expressed in fat cells of gliomas with fat cells components.CD133^＋blood vessels could be observed.ConclusionThe advance of tumor grade,was related to the increasing of CD15⁺ and CD133⁺ cells and their niches.CD15 and CD133 expression was positive correlation.The over-expression of CD133 in fat cells of glioma suggested a poor clinical prognosis.