| 转录因子T-bet和GATA-3对再生障碍性贫血免疫失衡的作用 |
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| 引用本文： | 杨晓红,唐旭东,许勇钢,张姗姗,麻柔,刘锋. 转录因子T-bet和GATA-3对再生障碍性贫血免疫失衡的作用[J]. 广东医学, 2010, 31(3) |
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| 作者姓名： | 杨晓红 唐旭东 许勇钢 张姗姗 麻柔 刘锋 |
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| 作者单位： | 中国中医科学院西苑医院血液科,北京,100091;中国中医科学院西苑医院血液科,北京,100091;中国中医科学院西苑医院血液科,北京,100091;中国中医科学院西苑医院血液科,北京,100091;中国中医科学院西苑医院血液科,北京,100091;中国中医科学院西苑医院血液科,北京,100091 |
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| 基金项目： | 国家自然科学基金资助项目(编号:30600837) |
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| 摘 要： | 目的探讨外周血中T-bet和GATA-3基因的表达在再生障碍性贫血(aplastic anemia,AA)中发病机制及意义。方法选择23例AA患者为再障组,其中重型再障(severe aplastic anemia,SAA)13例,轻型再障(mediate aplastic anemia,mAA)10例,再选择22例健康体检为对照组,对纳入对象采用流式细胞术检测其外周血Th1和Th2,同时运用RT-PCR技术检测外周血单个核细胞(PBMCs)中转录因子T-bet和GATA-3 mRNA基因表达。结果与对照组相比,再障组的lg(T-bet/β-actin)和Th1(%)升高,差异有统计学意义(P<0.05、P<0.01),lg(GATA-3/β-actin)和Th2(%)降低,差异有统计学意义(P<0.05、P<0.05);其中SAA组的lg(T-bet/β-actin)、lg(GATA-3/β-actin)和Th1(%)、mAA组的lg(T-bet/β-actin)、Th1(%)相比对照组明显升高(P<0.05、P<0.01、P<0.01、P<0.05、P<0.05);SAA组的Th2(%)、mAA组的lg(...
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| 关 键 词： | 再生障碍性贫血 T-bet/GATA-3 RT-PCR 转录因子 |
Expression of T-bet/GATA-3 mRNA in peripheral blood in patients with aplastic anemia |
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| YANG Xiao-hong,TANG Xu-dong,XU Yong-gang,Zhang Shan-shan,MA Rou,LIU Feng. Expression of T-bet/GATA-3 mRNA in peripheral blood in patients with aplastic anemia[J]. Guangdong Medical Journal, 2010, 31(3) |
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| Authors: | YANG Xiao-hong TANG Xu-dong XU Yong-gang Zhang Shan-shan MA Rou LIU Feng |
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| Affiliation: | YANG Xiao-hong,TANG Xu-dong,XU Yong-gang,Zhang Shan-shan,MA Rou,LIU Feng.National Hematological Medical Center of Traditional Chinese Medicine.Department of Hematology,Xiyuan Hospital affiliated to China Academy of Traditional Chinese Medicine,Beijing 100091,China |
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| Abstract: | Objective To investigate the pathogenic role of T-bet and GATA-3 mRNA expression in aplastic anemia(AA).Methods Twenty-three patients with AA(AA group) and 22 healthy people(control group) were included.Flow cytometry was used to detect the expression of Th1 and Th2 in peripheral blood and RT-PCR for quantifying the expression of transcription factor T-bet and GATA-3 mRNA.Results Compared with the control group,the expression of Th1 and T-bet mRNA in the AA group significantly increased(P<0.01,P<0.05),while... |
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| Keywords: | aplastic anemia T helper cell T-bet GATA-3 |
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