| 高侵袭性胰腺癌Panc-1细胞系中干细胞筛选结果分析 |
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| 引用本文： | 沈俊,王敏,刘文松,江建新,秦仁义. 高侵袭性胰腺癌Panc-1细胞系中干细胞筛选结果分析[J]. 山东医药, 2009, 49(26): 24-25 |
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| 作者姓名： | 沈俊 王敏 刘文松 江建新 秦仁义 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院,武汉,430032 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的探讨高侵袭性胰腺癌细胞中是否有比例较高的胰腺癌干细胞。方法采用Transwell小室肿瘤侵袭模型筛选、分离出胰腺癌细胞系Pane-1中的高侵袭性癌细胞（Pane-1-H）和低侵袭性癌细胞（Pane-1-L），FCM检测上述两种细胞的胰腺癌干细胞表面标记物CD34、CD44、ESA。结果Pane-1-H、Pane-1-L中胰腺癌干细胞所占比例相近，随着侵袭时间延长，胰腺癌干细胞比例无改变，P均〉0．05。结论高侵袭性胰腺癌细胞中不含有较高比例的胰腺癌干细胞。
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| 关 键 词： | 胰腺癌 肿瘤干细胞 肿瘤细胞侵袭性 |
Analyzing the result of screening out the pancreatic cancer stem cells from the high-invasive Panc-1 cell line |
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| SHEN Jun,WANG Min,LIU Wen-song,JIANG Jian-xin,QIN Ren-yi. Analyzing the result of screening out the pancreatic cancer stem cells from the high-invasive Panc-1 cell line[J]. Shandong Medical Journal, 2009, 49(26): 24-25 |
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| Authors: | SHEN Jun WANG Min LIU Wen-song JIANG Jian-xin QIN Ren-yi |
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| Affiliation: | (Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430032 ,P. R. China) |
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| Abstract: | Objective To study whether there is more pancreatic cancer stem cells in high-invasive pancreatic cancer cells. Methods The transwell cave tumor invasion model was applied to select high-invasive cancer cells and lower-invasive cancer cells in the Panc-1 pancreatic cancer cell lines. FCM was used to detect the expression of pancreatic cancer stem cell surface markers CD24, CD44, ESA in high-invasive pancreatic cancer cells and lower-invasive pancreatic cancer cells. Result The proportion of pancreatic cancer stem cells showed no differences in high-invasive pancreatic cancer cells and lower-invasive pancreatic cancer cells and did not change with the prolongation of invasion time, P 〉 0.05. Conclusion there is no more pancreatic cancer stem cells in high-invasive pancreatic cancer cells. |
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| Keywords: | pancreatic cancer cancer stem cell tumor cell invasion |
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