| 白细胞介素2的检测与大鼠白介素2的制备及部分纯化 |
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| 引用本文： | 成忆军 叶天星. 白细胞介素2的检测与大鼠白介素2的制备及部分纯化[J]. 第二军医大学学报, 1989, 10(1): 40-43,48 |
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| 作者姓名： | 成忆军 叶天星 |
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| 作者单位： | 第二军医大学免疫学教研室(成忆军，叶天星)，第二军医大学免疫学教研室(杨嗣坤) |
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| 摘 要： |
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| 关 键 词： | 白细胞介素2 大鼠 杀伤细胞 |
MICROASSAY,PREPARATION AND PARTIAL PURIFICATION OF RAT INTERLEUKSN 2 |
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| Chen Yijun,et al. MICROASSAY,PREPARATION AND PARTIAL PURIFICATION OF RAT INTERLEUKSN 2[J]. Former Academic Journal of Second Military Medical University, 1989, 10(1): 40-43,48 |
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| Authors: | Chen Yijun et al |
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| Affiliation: | Department of Immunology |
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| Abstract: | An interleukin 2 (IL-2) microassay based on IL-2 dependent cell line CTLL was established referring to Gillis'method.The data were transformed to probit unit, and the regression line obtained had a good correlation(r>0.99).The IL-2 activity of control sample of our laboratory was determined according to recom-binant human IL-2 standard,the ability of IL-2 induced by rat and mouse spleen cells was compared, and the parameters of IL-2 induced by rat spleen cells were detected.The partially purified rat IL-2 was obtained through ammonium sulfate precipitation, gel filtration and chromatography. |
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| Keywords: | interleukin 2 probit unit analysis induction purification |
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