低氧对鼠雪旺氏细胞增殖的影响 |
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引用本文: | 张振兴,宋小峰.低氧对鼠雪旺氏细胞增殖的影响[J].中国医疗前沿,2008,3(19):9-10. |
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作者姓名: | 张振兴 宋小峰 |
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作者单位: | 张振兴(辽宁医学院附属第一医院神经外科,辽宁锦州,121001);宋小峰(辽宁医学院组胚教研室,辽宁锦州,121001) |
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摘 要: | 目的观察低氧对鼠雪旺氏细胞体外增殖的影响,为雪旺氏细胞扩增培养探索新方法。方法原代分离培养雪旺氏细胞并鉴定,将培养至第三代的细胞分为3组,分别为对照组,10%02组和3%O2组。对各组细胞记数;测定培养液中氧含量和乳酸浓度及各组细胞的增殖指数。结果随着环境氧浓度的下降,细胞培养液中的氧含量也逐渐下降(P<0.05),而乳酸浓度逐渐升高(P<0.05)。低氧组雪旺氏细胞数目比常氧组明显增加,尤以10%O2组更为明显(P<0.05),3%O2组在低氧1d和2d时增殖指数低于常氧组,低氧4时,增殖指数增加;而10%O2组增殖指数一直高于常氧组(P<0.05)。结论轻中度低氧有利于鼠雪旺氏细胞的体外增殖。
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关 键 词: | 低氧 雪旺氏细胞 增殖 |
Effects of hypoxia on the proliferation of Schwann cells in vitro |
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Abstract: | Objective To investigate the effects of hypoxia on proliferation of Schwann cells and find a new way of expansion of Schwann cells in vitro. Methods Normal rat s sciatic nerves were used for the primary culture Schwann cells. Schwann cells were dissociated and divided into three groups(control,10%O2 and 3%O2)after identification.The numbers of Schwann cells were counted and the proliferation indexes of Schwann cells were detected by flow cytometry.The concentration of lactic acid and oxygen in the culture f... |
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Keywords: | hypoxia Schwann cells proliferation |
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