Polyamine analysis of infected root canal contents related to clinical symptoms

Abstract The contents of infected root canals were collected by mechanical and chemical debridement. Intracanal polyamines were identified and quantified by HPLC in all canals examined, however, no histamine was detected in any canal. The relationship between the amount of each polyamine and clinical signs of the teeth was analyzed statistically. The teeth without clinical signs tended to have limited varieties and smaller amounts of polyamines. Using %2, the amounts were significantly greater in teeth with spontaneous pain, swelling and putrescent odor (p<0.01), with exudate (p<0.025) and with percussion pain (p<0.1) than in teeth without. Amounts of putrescine (p<0.05) and total polyamines (p<0.01) were greater in teeth with spontaneous pain and percussion pain than in teeth without clinical sign, and those with root canal exudate also had greater amounts of total polyamines than those without (p<0.01). The amounts of cadaverine, however, from the teeth with gingival fistulae, was greater than from those without (p<0.05). No significant relationship was established between the amount of each polyamine and the presence of putrescent odor or gingival swelling. Intracanal polyamines, especially putrescine may leak out through apical foramen and may be implicated in pain production by eliciting acute inflammatory response in the periapical tissues.