补肾填精方对再障模型小鼠骨髓造血干（祖）细胞CFU—GM、CFU—E的影响

目的：观察补肾填精方对再生障碍性贫血（简称再障）模型小鼠骨髓造血功能的影响。方法：小鼠在接受给予环磷酰胺及苯油1周后每天经灌胃分别给予补肾填精方悬液3个剂量（20mg·kg^-1·d^-1、30mg·kg^-1·d^-1、40mg·kg^-1·d^-1）35天后眶动／静脉采血法检测外周血细胞数，取小鼠左侧股骨骨髓制备骨髓有核细胞悬液并进行有核细胞（BMNC）计数，甲基纤维素半固体法测定红系祖细胞集落（CFU—E）及粒单系祖细胞集落（CFU—GM）。结果：模型组小鼠红细胞（RBC）、血红蛋白（Hb）、白细胞（WBC）、血小板（PLT）、BMNC、CFU—E及CFU—GM数目较空白组明显减少（P〈0．01），中药中、高剂量组上述指标较模型组升高（P〈0．05）。结论：补肾填精方具有促进骨髓造血干／祖细胞造血，提升外周血细胞作用。

Effect of Formula of Invigorating the Kidney for Essence Replenishment on CFU-E and CFU-GM in Mice Model of Aplastic Anemia

Objective： To observe the effect of Formula of Invigorating the Kidney for Essence Replenishment on bone marrow haematogenesis in mice models of aplastie anemia. Method ： After 1 week of accepting eyclophosphamide and benzene oil, the mouse were given to different doses of Formula of Invigorating the Kidney for Essence Replenishment （20mg/kg · d, 30mg/kg · d and 40 mg/kg · d）. After 35 days peripheral blood cells were detected by blood collection method in arcula artery/vein, karyocyte was counted by taking mouse left femoral bone to prepare marrow karyocyte cell suspension, and CFU - E and CFU - GM were detected by semisolid cellog- ran method. Result： RBC, Hb, WBC, PLT, BMNC, CFU - E and CFU - GM were decreased in model group than in blank control group markedly （ P 〈 0. 01 ）. Above indicators in High and middle doses of treatment groups were increased than those in model group （ P 〈 0.05 ）. Conclusion ： Formula of Invigorating the Kidney for Es- sence Replenishment can promote haematogenesis in haemopoietic stem cell, and improve cytosis of peripheral blood cells.