| 固相时间分辨荧光免疫分析的标记技术及标记过程中的蛋白质测定 |
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| 引用本文： | 刘婷婷,宁志刚,赫文龙,李俊玲,张树恒,潘利华.固相时间分辨荧光免疫分析的标记技术及标记过程中的蛋白质测定[J].标记免疫分析与临床,2009,16(2):108-111. |
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| 作者姓名： | 刘婷婷 宁志刚 赫文龙 李俊玲 张树恒 潘利华 |
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| 作者单位： | 长春工业大学,吉林,长春,130012;中国科学院长春应用化学研究所,国家电化学光谱分析研究中心,吉林,长春,130022 |
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| 摘 要： | 利用新型双功能螯合剂-4，7-二氯磺酰基苯基-1，10-菲啰啉-2，9-二羧酸（BCPDA）进行固相时间分辨荧光免疫分析（TRFIA）标记技术研究，结合标记牛血清白蛋白（BSA）实验，研究测定蛋白质方法，监测标记过程，计算回收率、标记比。确定考马斯亮蓝染色法为该体系标记研究中测定蛋白质含量方法。
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| 关 键 词： | 时间分辨荧光免疫分析 BCPDA 蛋白质测定 考马斯亮蓝染色法 |
Determination of Protein in the Labeling Process of Time-resolved Fluoroimmunoassay |
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| LIU Ting-ting,NING Zhi-gang,HE Wen-long,LI Jun-ling,ZHANG Shu-heng,PAN Li-hua.Determination of Protein in the Labeling Process of Time-resolved Fluoroimmunoassay[J].Labeled Immunoassays and Clinical Medicine,2009,16(2):108-111. |
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| Authors: | LIU Ting-ting NING Zhi-gang HE Wen-long LI Jun-ling ZHANG Shu-heng PAN Li-hua |
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| Institution: | LIU Ting-ting, NING Zhi-gang, HE Wen-long, LI Jun-ling, ZHANG Shu-heng, PAN Li-hua (Changchun University of Technology, Changchun 130012, China) |
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| Abstract: | A new type of bifunctional chelating agent 4,7-bis-chlorosulfophenyl-1, 10-Phenanthroline-2,9-dicarboxylic acid （BCPDA） was used in the labeling study of time-resolved fluoroimmunoassay （TRFIA）. The method of protein measurement, inspection of labeling process and recovering yield in the labeling were carried out by using blood serum protein （BAS） labeling experiment. The results showed that the coloration method of coomassie blue staining might be used to determine the protein content in this labeling process of TRFIA. |
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| Keywords: | BCPDA |
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