多孔纳米羟基磷灰石/胶原复合材料的制备及性能

目的：探讨多孔纳米羟基磷灰石/胶原复合材料（NHAC）简单有效的复合方法和结构及其作为新型骨替代材料应用于组织工程的可行性。方法：以氢氧化钙和磷酸为原料合成多孔纳米羟基磷灰石晶体，从牛肌腱中提取可溶性I型胶原，应用戊二醛交联冻干法使二者复合制备成生物膜，用XRD和扫描电镜，对材料晶相组成、微观形貌、结构、晶粒大小进行分析。 结果：通过对合成工艺研究，制得的生物膜呈三维网孔状结构，孔径10～40 μm，大小均匀，由纳米尺度（10 nm×50 nm~20 nm×80 nm）的羟基磷灰石和胶原纤维组成。复合材料在晶相组成和尺寸上类似于天然骨。结论：NHAC复合材料有望成为理想的骨替代材料。

Preparation of porous nano-hydroxyapatite/collagen composite and its capability

Objective To study the simple preparation method and structure of nano-hydroxyapatite/collagen composite, to investigate new substitute of repairing bone for using in tissue engineering. Methods Porous nano-hydroxyapatite was made of Ca (OH)2 and H3PO4. Collagen was drawn from fresh adult bovine tendon. The two materials were prepared into biomembrane through the glutaraldehyde and freeze-drying. The crystallite phase, micro-morphology, structure, crystallite size of the composite were examined by XRD and scanning electronic microscop (SEM). Results The results showed that the composite structure was porous and consisted of nano-hydroxyapatite (10 nm × 50 nm - 20 nm × 80 nm) and collagen fiber. The crystallite phases and size of the composite was similar to that of natural bone. Conclusion The porous nano-hydroxyapatite /collagen composite is expected to be an ideal substitute of repairing bone.