| MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer’s Disease |
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| 作者姓名： | LIU Chen Geng MENG Shuang LI Ying LU Yao ZHAO Yue WANG Pei Chang |
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| 作者单位： | Clinical Laboratory of Xuanwu Hospital, Captital Medcial University, Beijing 100053, China;State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Beijing 102206, China;Clinical Laboratory of Air Force General Hospital, Chinese People's Liberation Army, Beijing 100142, China;Clinical Laboratory of Xuanwu Hospital, Captital Medcial University, Beijing 100053, China;Clinical Laboratory of Xuanwu Hospital, Captital Medcial University, Beijing 100053, China;Clinical Laboratory of Xuanwu Hospital, Captital Medcial University, Beijing 100053, China |
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| 基金项目： | No. 81401734, 81472007;No. 2016YFC1306300;the National Key Research and Development Program of China;This study was supported by the National Natural Science Foundation of China |
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| 摘 要： | Objective In the present study,the ABCA1 was used as a label to capture specific exosomes,the level of ABCA1-labeled exosomal microRNA-135 a(miR-135 a)was evaluated for the diagnosis of Alzheimer’s disease(AD),especially in patients with early stages of AD.Methods This is a preliminary research focused on the levels of ABCA1 in WBCs,RBCs,HT-22 cells,and neuron cells.The diagnostic value of ABCA1-labeled exosomal miR-135 a was examined using the CSF and serum of APP/PS1 double transgenic mice,and 152 patients with SCD,131 patients with MCI,198 patients with DAT,and 30 control subjects.Results The level of ABCA1 exosomes harvested from HT-22 cells and neuron culture medium was significantly higher compared to that of RBCs and WBCs(P<0.05).The levels of ABCA1-labeled exosomal miR-135 a increased in the CSF of MCI and DAT group compared to those of control group(P<0.05),slightly increased(P>0.05)in the serum of SCD patient group,and significantly increased in MCI and DAT patient groups compared to those of the control group(P<0.05).Conclusion This study outlines a method to capture specific exosomes and detect them using immunological methods,which is more efficient for early diagnosis of AD.
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| 关 键 词： | Alzheimer’s disease EXOSOME MICRORNA BIOMARKER |
| 收稿时间： | 7 March 2020 |
MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer's Disease |
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| LIU Chen Geng,MENG Shuang,LI Ying,LU Yao,ZHAO Yue,WANG Pei Chang.MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer's Disease[J].Biomedical and Environmental Sciences,2021,34(1):19-28. |
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| Authors: | LIU Chen Geng MENG Shuang LI Ying LU Yao ZHAO Yue WANG Pei Chang |
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| Institution: | 1. Clinical Laboratory of Xuanwu Hospital, Captital Medcial University, Beijing 100053, China;2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Beijing 102206, China;3. Clinical Laboratory of Air Force General Hospital, Chinese People''s Liberation Army, Beijing 100142, China |
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| Abstract: | Objective In the present study, the ABCA1 was used as a label to capture specific exosomes, the level of ABCA1-labeled exosomal microRNA-135a (miR-135a) was evaluated for the diagnosis of Alzheimer’s disease (AD), especially in patients with early stages of AD. Methods This is a preliminary research focused on the levels of ABCA1 in WBCs, RBCs, HT-22 cells, and neuron cells. The diagnostic value of ABCA1-labeled exosomal miR-135a was examined using the CSF and serum of APP/PS1 double transgenic mice, and 152 patients with SCD, 131 patients with MCI, 198 patients with DAT, and 30 control subjects. Results The level of ABCA1 exosomes harvested from HT-22 cells and neuron culture medium was significantly higher compared to that of RBCs and WBCs (P < 0.05). The levels of ABCA1-labeled exosomal miR-135a increased in the CSF of MCI and DAT group compared to those of control group (P < 0.05), slightly increased (P > 0.05) in the serum of SCD patient group, and significantly increased in MCI and DAT patient groups compared to those of the control group (P < 0.05). Conclusion This study outlines a method to capture specific exosomes and detect them using immunological methods, which is more efficient for early diagnosis of AD. |
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| Keywords: | Alzheimer's disease Exosome MicroRNA Biomarker |
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