大孔树脂与活性炭富集山茱萸总苷的实验研究

目的:比较大孔树脂与活性炭富集山茱萸总苷的工艺条件及参数。方法:以山茱萸总苷中主要有效成分莫诺苷及马钱素为考察指标,用HPLC法观察大孔树脂及活性炭富集山茱萸总苷的最佳工艺条件。结果:山茱萸提取液10 ml上大孔树脂及活性炭柱(R25mm×H300mm),先用蒸馏水洗脱除去水溶性杂质,再用乙醇梯度洗脱。结果表明以30％及50％乙醇洗脱大孔树脂柱、以30％、50％、70％乙醇洗脱活性炭柱为最佳分离工艺。结论:大孔树脂富集山茱萸总苷优于活性炭,前者对莫诺苷和马钱素的洗脱率约为96％,后者约为85％。故大孔树脂可作为相关制剂富集山茱萸总苷的首选。

Study on Enrichment Process of Cornus o fficinalis Glycosides with Macroporous Resin and Active Carbon

Objective: To study the technological parameters of the enrichment purification of Cornus officinalis glycosides (COG) with macroporous resin and active carbon. Methods: COG was isolated and purified by active carbon and macroporous resin HPD100. The enrichment of COG was evaluated by HPLC method. Results: The optimum process was that 10ml extract of Fructus Corni was absorbed with columns of macroporous resin and active carbon(R25 mm x H300 mm) and the columns were washed with 600ml distilled water, then the morroniside and loganin was eluted from the macroporous resin and active carbon with ethanol. Conclusion: The elutive rate of morroniside and logann was about 85% by the macroporous resin,96% by the active carbon. So this method enriching COG from Fructus Corni is practical.