磁示踪技术用于胃肿瘤标记定位的实验研究

目的通过动物实验验证基于磁示踪技术的胃肿瘤标记定位的安全性和可行性。方法选取6只雄性健康Beagle犬,全麻后行胃镜检查,假定胃肿瘤部位,然后利用内镜下软组织夹将示踪磁体钳夹并固定于胃肿瘤部位,退出胃镜。24 h后再次全麻,行腹腔镜胃肿瘤定位。常规建立腹腔镜戳孔并进镜后经操作孔置入寻踪磁体,将寻踪磁体置于胃表面肿瘤附近探査示踪磁体,两个磁体相吸后,腹腔镜下所见寻踪磁体所在部位即为胃肿瘤的位置,从而完成病灶的标记和定位。结果6只Beagle犬在胃镜下均成功留置示踪磁体,胃镜操作中无磁体滑脱、副损伤、出血等。胃镜术后24 h,在腹腔镜手术下顺利置人寻踪磁体,寻踪磁体与示踪磁体自动相吸,形成“示踪磁体-胃壁-寻踪磁体”的三明治样结构,完成腹腔镜下对胃肿瘤的定位和识别,整个过程操作顺利,未出现意外损伤。结论基于磁示踪技术的胃镜联合腹腔镜胃肿瘤标记定位操作简单、定位准确、安全可行。

Experimental study of stomach tumor localization based on magnetic tracer technique

Objective To verify the feasibility and safety of stomach tumor marker localization based on magnetic tracer technique in dogs.Methods Six male Beagle dogs were examined by gastroscopy.Then tracer magnets were sent to the“tumor”locations assumed in advance and fixed near the“tumors”by endoscopic soft tissue clamp.Laparoscopic gastric tumor localization was performed under general anesthesia 24 hours later.The tracer magnet was placed near the tumor on the surface of the stomach through the operating hole after the conventional establishment of laparoscope puncture parallel mirror to explore the tracer magnet.After the two magnets were attracted,the location of the tracer magnet seen under the laparoscope was the location of the gastric tumor,so as to complete the labeling and positioning of the lesion.Results All the 6 Beagle dogs were successfully implanted with tracer magnets under gastroscopy.Twentyfour hours after the gastroscopy,the pursuit magnet was successfully implanted during laparoscopic surgery.The two magnets automatically attracted each other and formed a sandwich structure of “tracer magnet-gastric wall-pursuit magnet”,which completed the location and identification of gastric tumor under the laparoscopy.Conclusion Gastroscopy combined with laparoscopy based on magnetic tracer technique is simple,accurate,safe and feasible.