Benzo(a)pyrene metabolism in hepatic microsomes from female DA rats with a genetic sparteine oxidation deficiency |
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| Authors: | E Schlede I Roots |
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| Affiliation: | 1. Max von Pettenkofer Institut, Bundesgesundheitsamt, BerlinFRG;1. Institut für Klinische Pharmakologie, Klinikum Steglitz, Freie Universität Berlin, Hindenburgdamm 30, D-1000 Berlin 45, F.R.G. |
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| Abstract: | The impact of the genetic hydroxylation deficiency described for several drugs such as sparteine, debrisoquine, and phenformin, has been studied with respect to benzo(a)pyrene (BP) metabolite formation. 14C-BP (80 microM) was incubated with liver microsomes from female DA rats deficient in sparteine oxidation; microsomes from female Sprague-Dawley rats served as controls. BP metabolites were separated by high pressure liquid chromatography (HPLC). No significant differences were detected in the overall formation rate of 9,10-,4,5-, and 7,8-dihydrodiol-BP, of 4 quinones, or of 9-OH- and 3-OH-BP. Thus, the study suggested no association between genetic hydroxylation polymorphism (debrisoquine/sparteine type) and the formation of at least 6 BP metabolites. |
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| Keywords: | Benzo(a)pyrene metabolism genetic hydroxylation polymorphism (sparteine/ debrisoquine type) pharmacogenetics DA rat cytochrome P-450 AHH aryl hydrocarbon hydroxylase BP benzo(a)pyrene HPLC high pressure liquid chromatography |
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