川芎嗪对染矽尘大鼠肺组织Ⅰ、Ⅲ型胶原合成的影响

目的观察川芎嗪对染矽尘大鼠肺组织Ⅰ、Ⅲ型胶原合成的影响。方法将大鼠随机分为对照组、染矽尘组、川芎嗪组,采用一次性气管内注入矽尘法建立矽肺动物模型,川芎嗪组每日腹腔注射川芎嗪注射液50mg/kg。染尘后按不同时间点取大鼠肺组织制作石蜡切片,采用天狼猩红染色结合偏振光显微镜观察Ⅰ、Ⅲ型胶原的表达,ImageProPlus图像分析系统进行定量分析。结果染矽尘后第3天,Ⅲ型胶原开始增生,染矽尘后第7天,Ⅰ型胶原开始增生,直至染矽尘后第28天,这两种胶原进行性增加。与对照组相比,各时间点染矽尘组大鼠肺组织Ⅰ、Ⅲ型胶原面积百分比均有不同程度增加,而川芎嗪组Ⅰ型胶原面积百分比在第7、14、28天,Ⅲ型胶原面积百分比在第28天时明显低于染矽尘组。结论矽尘可以诱导大鼠肺组织Ⅰ、Ⅲ型胶原合成增加,两型胶原出现增生的时相有所不同;川芎嗪可以抑制矽尘诱导的Ⅰ、Ⅲ型胶原的合成。

Effect of Ligustrazine on type Ⅰ, Ⅲ collagen synthesis in lung tissue of silica-treated rat

OBJECTIVE: To study the effect of Ligustrazine on type I, III collagen synthesis in lung tissue of silica-treated rat. METHODS: The 128 experimental rats were randomly divided into control, silica and Ligustrazine group. 1ml silica (50g/L) was injected intratracheally in silica group and Ligustrazine group, while Ligustrazine group were injected intraperitoneally 50mg/(kg x d) Ligustrazine. Samples were collected on the 1st, 3rd, 7th, 14th, 21st, 28th day after injected silica. Type I , III collagen on paraffin-embedded lung sections were stained with sirius red, detected by polarized light microscopy and quantified by Image-Pro Plus. RESULTS: On the 3rd day after silica instillation, type III collagen began to appear, while type I collagen formed on the 7th day. Thereafter Type I, III collagen increased progressively. Compared with control group, type I, III collagen expression of silica group increased. At different time points type I, III collagen expression of Ligustrazine group decreased than silica group. There was significant difference of type I collagen area percentage on the 7th, 14th, 28th day, type III collagen area percentage on the 28th day between Ligustrazine group and silica group. CONCLUSION: Silica dioxide could induce the increase of type I, III collagen production in lung tissue, and their stages are different. Ligustrazine could suppress type I, III collagen production in lung tissue of silica-treated rat, and it's mechanism would be studied.