深低温停循环间断灌注脑保护液的实验研究

目的 研究深低温停循环（ＤＨＣＡ）间断灌注充氧脑保护液的脑保护效果及机制。方法 健康杂种犬１０条，Ａ组单纯ＤＨＣＡ１２０ｍｉｎ，Ｂ组ＤＨＣＡ后经双侧颈动脉间断灌注充氧脑保护液。两组动物于不同时相取皮层组织测定其三磷酸腺苷（ＡＴＰ）的含量及一氧化氮合酶（ＮＯＳ）的生，并作透射电镜（ＴＥ）检查。结果 ＤＨＣＡ１２０ｍｉｎ及恢复循环４５ｍｉｎ后，Ｂ组皮层组织ＡＴＰ的含量均明显高于Ａ组。Ａ组皮层组织还原型

Experimental study of cerebral protection of intermittent cerebroplegia perfusion during deep hypothermic circulatory arrest

Objective\ To study the protective effect and the protective mechanism of intermittent oxygenated cerebroplegia perfusion during deep hypothermic circulatory arrest (DHCA). Methods\ Ten mongrel dogs underwent DHCA for 120 min. Five dogs in group B received oxygenated cerebroplegic infusion and 5 dogs in group A did not following DHCA. Cerebral cortex was collected at different time points to determine ATP content, NOS activity and examine the ultrastructure under transmission electron microscopy. Results\ At 120 min of DHCA and at 45 min of reperfusion, the content of ATP in the group B was higher significantly than in the group A. The number of NOS positive neurons in the group A was higher significantly than in the group B. TE examination revealed that the damage of the ultrastructure in the group A was more severe than in the group B. Conclusion\ Intermittent oxygenated cerebroplegia perfusion during DHCA is an optimal cerebral protective method.