C57-ras转基因小鼠模型杂交1代CB6F1的生长发育特性

目的 测定自主建立的转基因致癌性模型C57-ras小鼠与BALB/c 小鼠杂交1 代的生长发育特性参数, 为该模型的商业化供应提供基础数据。方法 将雄性转基因阳性C57-ras小鼠与野生型雌性BALB/c 小鼠交配, 获得杂交1 代(CB6F1 c-Ha-ras^+/-)并测定平均产仔数、离乳率、阳性鼠生长发育情况、转基因阳性率等参数。结果 在生长发育方面, 雌鼠平均每窝产子数为8只, 离乳率为90%, 雄雌性别比符合预期。CB6F1的平均出生重为(1.73±0.05)g, 10周龄时阳性鼠平均体重雌性(24.38±1.74)g, 雄性(29.42±1.72)g。雄性鼠体重明显高于雌性, 差异存在极显著性(P<0.01);基因型检测阳性率为46.9%, 符合遗传规律。结论 转基因致癌性模型C57-ras小鼠与BALB/c 小鼠杂交1 代的生长发育特性正常, 可实现规模化商业供应。

Characterization of Growth Performance of F1 hybrid CB6F1 C57-ras Transgenic Mice Model

Objective To obtain the basic growth parameters of a self-established F1 hybrid CB6F1 C57-ras transgenic mouse model, and to provide basic information for commercialization of this mouse model. Methods F1 hybrid mice (CB6F1) were produced by crossing C57-ras heterozygous transgenic (c-Ha-ras^+/-) male mice and wild-type BALB/cJ female mice. The average litter size, weaning rate, sex ratio, growth performance and C57-ras transgenic positive rate were recorded and analyzed. Results The average litter size was eight, weaning rate was 90%, and sex ratio was approximately in accordance with prediction. The average body weight of newborn mice was 1.73±0.05 g. The average body weight of 10-week old c-Ha-ras transgenic female and male mice in CB6F1 background was 24.38±1.74 g and 29.42±1.72g, respectively, which had a significant difference (P<0.01). The c-Ha-ras transgenic positive rate was 46.9%. which was in accordance with genetic rules. Conclusions The F1 hybrid mice (CB6F1) produced by crossing C57-ras heterozygous transgenic (c-Ha-ras +/-) male mice and wild-type BALB/cJ female mice show normal growth performance and development characteristics, and it can be used for large-scale commercial supply.