探讨荧光定量PCR在结核菌检测中的应用 |
| |
引用本文: | 李洪敏,王治伟,张霞,苗青,刘银萍. 探讨荧光定量PCR在结核菌检测中的应用[J]. 临床肺科杂志, 2008, 13(11): 1436-1438 |
| |
作者姓名: | 李洪敏 王治伟 张霞 苗青 刘银萍 |
| |
作者单位: | 北京解放军总医院第二临床部结研所临床试验室,100091;北京解放军总医院第二临床部结研所临床试验室,100091;北京解放军总医院第二临床部结研所临床试验室,100091;北京解放军总医院第二临床部结研所临床试验室,100091;北京解放军总医院第二临床部结研所临床试验室,100091 |
| |
摘 要: | 目的探讨荧光定量PCR技术在检测临床标本中结核分枝杆菌的应用价值。方法通过荧光定量PCR技术鉴定350株临床分离株,进行结核分枝杆菌的菌种鉴定和定量分析,同时进行抗酸染色和培养法。结果荧光定量PCR、染色和培养法阳性检出率分别为38.8%(128/330),21.8%(72/330),29.7%(98/330),提高阳性检出率(涂阴)16.97%(56/330),菌种鉴定特异性实验符合率为100%。结论荧光定量PCR技术是一种快速、敏感、特异性强的结核分枝杆菌辅助诊断方法。
|
关 键 词: | 结核分枝杆菌 荧光定量PER 定量分析 菌种鉴定 |
Real time-FQ-PCR Application in the Detection and Study of M.tuberculosis |
| |
Affiliation: | LI Hong-min, WANG Zhi-wei, ZHANG Xia ,et al. (2th Clinical Division of Chinese PLA General Hospital Tuberculosis clinical Lab Beijing 100091) |
| |
Abstract: | Objective Discusses Real time-FQ-PCR application value in the detection and study of clinical specimen of M tuberculosis. Method Identification strain and real-time analysis of M. tuberculosis by identified 350 Clinical Isolated Strains of M. tuberculosis with Real time-FQ-PCR,acid-fast staining and culture had been done as contrast control at the same time. Result The detection rates of Real time-FQ-PCR,staining and culture is 38.8% (128/330) ,21.8% (72/330) ,29.7% (98/330) respectively. The detection rates was been increased 16. 97% (56/330) high than staining,the coincidence rate of the specificity of identification strain by real time-FQ-PCR was 100%. Conclusion Real time-FQ-PCR is a fast,sensitive and specificity method in auxiliary diagnosis of M. tuberculosis. |
| |
Keywords: | M tuberculosis Real time-FQ-PCR quantitative analysis strain identification. |
本文献已被 维普 万方数据 等数据库收录! |
|