| 探讨荧光定量PCR在结核菌检测中的应用 |
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| 引用本文： | 李洪敏,王治伟,张霞,苗青,刘银萍. 探讨荧光定量PCR在结核菌检测中的应用[J]. 临床肺科杂志, 2008, 13(11): 1436-1438 |
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| 作者姓名： | 李洪敏 王治伟 张霞 苗青 刘银萍 |
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| 作者单位： | 北京解放军总医院第二临床部结研所临床试验室,100091;北京解放军总医院第二临床部结研所临床试验室,100091;北京解放军总医院第二临床部结研所临床试验室,100091;北京解放军总医院第二临床部结研所临床试验室,100091;北京解放军总医院第二临床部结研所临床试验室,100091 |
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| 摘 要： | 目的探讨荧光定量PCR技术在检测临床标本中结核分枝杆菌的应用价值。方法通过荧光定量PCR技术鉴定350株临床分离株，进行结核分枝杆菌的菌种鉴定和定量分析，同时进行抗酸染色和培养法。结果荧光定量PCR、染色和培养法阳性检出率分别为38．8％（128／330），21．8％（72／330），29．7％（98／330），提高阳性检出率（涂阴）16．97％（56／330），菌种鉴定特异性实验符合率为100％。结论荧光定量PCR技术是一种快速、敏感、特异性强的结核分枝杆菌辅助诊断方法。
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| 关 键 词： | 结核分枝杆菌 荧光定量PER 定量分析 菌种鉴定 |
Real time-FQ-PCR Application in the Detection and Study of M.tuberculosis |
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| Affiliation: | LI Hong-min, WANG Zhi-wei, ZHANG Xia ,et al. (2th Clinical Division of Chinese PLA General Hospital Tuberculosis clinical Lab Beijing 100091) |
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| Abstract: | Objective Discusses Real time-FQ-PCR application value in the detection and study of clinical specimen of M tuberculosis. Method Identification strain and real-time analysis of M. tuberculosis by identified 350 Clinical Isolated Strains of M. tuberculosis with Real time-FQ-PCR,acid-fast staining and culture had been done as contrast control at the same time. Result The detection rates of Real time-FQ-PCR,staining and culture is 38.8% （128/330） ,21.8% （72/330） ,29.7% （98/330） respectively. The detection rates was been increased 16. 97% （56/330） high than staining,the coincidence rate of the specificity of identification strain by real time-FQ-PCR was 100%. Conclusion Real time-FQ-PCR is a fast,sensitive and specificity method in auxiliary diagnosis of M. tuberculosis. |
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| Keywords: | M tuberculosis Real time-FQ-PCR quantitative analysis strain identification. |
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