The role of astrocytes in oxidative stress of central nervous system: A mixed blessing |
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| YUAN Shuwen, DONG Yiwei, LIU Jian, LIANG Yajie, HUANG Jianjun, XIAO Baoguo, WANG Qing, MA Cungen. Protective Effect and Mechanism of Proanthocyanidin B2 Against H2O2-induced Oxidative Damage and Apoptosis of Astrocytes[J]. Chinese Journal of Modern Applied Pharmacy, 2024, 41(6): 727-735. DOI: 10.13748/j.cnki.issn1007-7693.20230642 |
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| Authors: | YUAN Shuwen DONG Yiwei LIU Jian LIANG Yajie HUANG Jianjun XIAO Baoguo WANG Qing MA Cungen |
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| Affiliation: | 1.The Key Research Laboratory of Benefiting Qi for Acting Blood Circulation Method to Treat Multiple Sclerosis of State Administration of Traditional Chinese Medicine, Research Center of Neurobiology, Shanxi University of Chinese Medicine, Jinzhong 030619, China;2.The Key Laboratory of Prevention and Treatment of Neurological Disease of Shanxi Provincial Health Commission/Department of Neurosurgery, Sinopharm Tongmei General Hospital, Datong 037003, China;3.State Key Laboratory of Neurobiology, Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200025, China |
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| Abstract: | OBJECTIVE To investigate the protective effect proanthocyanidin B2(PC-B2) on oxidative damage and apoptosis of mouse astrocytes(AS) induced by hydrogen peroxide(H2O2) and its mechanism. METHODS AS were isolated and cultured from neonatal C57BL/6 mice(1−3 d). The optimal concentration of H2O2 and PC-B2 was divided into four groups: normal group, normal+PC-B2 group(100 μg·mL‒1 PC-B2 treated for 24 h), H2O2 model group(200 μmol·L‒1 H2O2 treated for 24 h), PC-B2 group(200 μmol·L‒1 H2O2 and 100 μg·mL‒1 PC-B2 treated for 24 h). The cell viability of each group was detected by CCK-8 method. Cytotoxicity was detected by LDH method. The antioxidant capacity was detected by ABTS and DPPH. The content of MDA and the activity of SOD, CAT and GSH-Px were detected by ELISA kit. Detection of apoptosis in each group was done by TUNEL staining. The mRNA and protein expression levels of Bax, Bcl-2, Caspase-3, Akt/Stat3, p-Akt, p-Stat3 and Nrf2/HO-1 in AS were detected by RT-PCR and Western blotting, respectively. RESULTS PC-B2 could significantly enhance cell viability and inhibit AS apoptosis. Compared with the H2O2 model group, PC-B2 intervention could significantly reduce the content of LDH and MDA in AS, and increase the activity of SOD, CAT and GSH-Px. PC-B2 intervention could inhibit the mRNA and protein expression of Bax and Caspase-3, and up-regulate the mRNA and protein expression of Akt/Stat3, Bcl-2, Nrf2/HO-1. CONCLUSION PC-B2 can enhance the antioxidant capacity of AS through Akt/Stat3 and Nrf2/HO-1 pathways, therefore reduce H2O2-induced AS oxidative damage and apoptosis. |
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| Keywords: | proanthocyanidin B2 astrocytes H2O2 oxidative damage cell apoptosis Akt/Stat3 Nrf2/HO-1 |
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