层次分析联合Box-Behnken响应面法优选复方肉苁蓉合剂提取工艺

目的 优选复方肉苁蓉合剂的提取工艺。方法 基于单因素试验，选择料液比、提取时间和浸泡时间为自变量，以毛蕊花糖苷、党参炔苷和丹酚酸B含量的综合评分为响应值，应用层次分析法（analytic hierarchy process，AHP）确定各权重系数，采用3因素3水平的Box-Behnken响应面法确定最佳提取工艺。结果 复方肉苁蓉合剂的最佳提取工艺为液料比10：1，浸泡1.8 h，提取2次，每次3.0 h。在此条件下，3种指标成分含量的综合评分为95.960 3，与模型预测值接近。结论 AHP确定的权重系数客观、真实，可以反映处方配伍信息；优选得到的工艺简便、稳定可行，可为复方肉苁蓉合剂的批量生产提供参考。

Optimization of Extraction Process for Compound Roucongrong Mixture by Analytic Hierarchy Process Combined with Box-Behnken Response Surface Methodology

OBJECTIVE To optimize the extraction technology of Compound Roucongrong mixture. METHODS Based on single factor experiments, set liquid-to-material ratio, extraction time and soaking time as independent variable, and the comprehensive score of the content of verbascoside, lobetyolin and salvianolic acid B as the response value. The 3-factor 3-level Box-Behnken response surface methodology was used to determine the optimal extraction process, and analytic hierarchy process(AHP) was adopted to determine the weight coefficient. RESULTS The optimal extraction technology was as follows: liquid-to-material ratio was 10：1, soaking for 1.8 h, extracting twice and 3.0 h each time. Under the optimum extraction technology conditions, comprehensive score was 95.960 3, which was closed to the predictive value of the model. CONCLUSION The weight coefficient determined by AHP is objective, real and can reflect the compound compatibility information. The optimized extraction technology is simple, convenient, stable and feasible, which can provide reference for mass production of Compound Roucongrong mixture.