miR-21、miR-182、miR-382在非小细胞肺癌中的临床意义及益气除痰法的干预作用

[目的]分析miR-21、miR-182、miR-382基因对肺癌进展的影响及益气除痰法的干预作用.[方法]应用生存分析在线软件Kaplan-Meier plotter分析miR-21、miR-182、miR-382对肺癌预后的影响,应用功能富集分析工具FunRich数据库对miR-21、miR-182、miR-382...

Clinical Significance of miR-21,miR-182 and miR-382 in Non-Small Cell Lung Cancer and Intervention Effect of Qi-Replenishing and Phlegm-Removing Therapy

Objective To analyze the effects of miR-21,miR-182,miR-382 genes on the progression of lung cancer and the intervention effect of qi-replenishing and phlegm-removing therapy.Methods The effects of miR-21,miR-182 and miR-382 on prognosis of lung cancer were analyzed by online software Kaplan-Meier plotter,the functional enrichment and pathway enrichment of miR-21,miR-182 and miR-382 were analyzed by FunRich database,and the interaction network of target gene proteins was analyzed by GeneMANIA database.We selected the non-small-cell lung cancer(NSCLC)patients received Chinese herbal medicine alone(served as Chinese medicine group)and the NSCLC patients received Chinese herbal medicine combined with chemotherapy(served as combination group),Quantitative polymerase chain reaction(PCR)was used to analyze the effect of qireplenishing and phlegm-removing therapy on the expression of miR-21,miR-182 and miR-382 in peripheral blood of the patients.Results The expression level of miR-21 and miR-382 was negatively correlated with the survival period of lung adenocarcinoma,but had no obvious correlation with the survival period of lung squamous cell carcinoma.The expression level of miR-182 had no significant correlation with the survival period of lung adenocarcinoma,but was positively correlated with survival of patients with lung squamous cell carcinoma.The functions of target genes of miR-21,miR-182,miR-382 were mainly related to signal transduction and intercellular communication,and the involved signal pathways mainly included integrin family signal pathway,vascular endothelial growth factor(VEGF)/vascular endothelial growth factor receptor(VEGFR)pathway,necrosis factor-related apoptosis-inducing ligand(TRAIL)pathway.The miR-21 target genes such as RECK,TNFRSF11B,TGFBI,PDCD4,SPRY2,FOXF2 was associated with tumor metastasis.The increase level of miR-21 in the Chinese medicine group was lower than that in the combination group.Conclusion miR-21 has possible effects on tumor metastasis and survival of lung adenocarcinoma.Qi-replenishing and phlegm-removing therapy is possibly effective for treatment of non-small-cell lung cancer by regulating expression of miR-21.