OmpA基因表达与鲍曼不动杆菌耐药的相关性

目的 分析鲍曼不动杆菌(Acinetobacter baumannii,AB)OmpA基因缺失株(△OmpA)和OmpA基因补救株(+OmpA)药物敏感性的差异,探讨临床株鲍曼不动杆菌OmpA mRNA表达水平与耐药的相关性,明确OmpA在鲍曼不动杆菌耐药中的作用.方法 采用定点诱变技术构建ATCC17978 OmpA...

The correlation between OmpA expression and drug resistance of Acinetobacter baumannii

Objective To analyze the differences in drug sensitivity between the OmpA gene deletion strain and the OmpA gene recovery strain, to explore the correlation between OmpA mRNA expression level and drug resistance of Acinetobacter baumannii clinical isolates, and to definitude the role of OmpA in drug resistance of A. baumannii. Methods ATCC17978 OmpA gene deletion and recovery strains were constructed by site-directed mutagenesis technology. Samples from 40 patients with infections of A. baumannii were collected at Fujian Longyan First Hospital from September 2020 to December 2020, then isolated and cultured the A. baumannii strains from clinical specimens. Fluorescence quantitative PCR was used to detect the OmpA mRNA expression level of clinical A. baumannii strains, and the sensibility test of 40 A. baumannii was determined by microdilution quantitative minimum inhibitory concentration (MIC) method, the American Clinical & Laboratory Standards Institute (CLSI) 2018 judgment standard was used to determine the drug sensitivity of A. baumannii strains. Results Deletion of the ompA gene led to decreased MICs of amikacin, cefepime, moxixaxil, imipenem, and piperacillin/tazobactam. In this study, 20 non-resistant clinical strains and 20 multi-drug resistant clinical strains were selected. Among them, 18 were resistant to amikacin and 22 were not resistant; 20 were resistant to cefepime and 20 were not resistant; 19 were resistant to imipenem and 21 were not resistant; 20 strains of piracillin and tazobactam were resistant and 20 were non-resistant. And the expression level of OmpA mRNA in multi-drug resistant A. baumannii strains was significantly higher than that in non-drug resistant strains (P<0.05). Conclusion The deletion of OmpA gene led to decreased MICs of amikacin, cefepime, moxixaxil, imipenem, and piperacillin/tazobactam. And the high expression of OmpA mRNA is a main factor for the drug resistance in the clinical isolates of A. baumannii in this institution. To monitor the OmpA mRNA expression level of A. baumannii clinical isolates can effectively predict the drug resistance of the strains, keep abreast of the epidemic dynamics about drug-resistant strains, effectively guide rational use of antibiotics in clinical treatment and hospital infection prevention.