云南省丽江市古城区鼠疫疫源地噬菌体的分离及鉴定

目的 调查丽江市古城区野鼠鼠疫疫源地宿主动物携带鼠疫噬菌体情况,并探讨其流行病学意义,为野鼠鼠疫疫源地宿主动物的防制提供科学依据.方法 2017年9月于丽江市古城区5个乡镇共13个野鼠鼠疫疫源地的鼠疫流行自然村采用5 m夹线法进行捕鼠,以鼠疫疫苗株EV76为饲养菌(即鼠疫噬菌体的宿主菌),采用双层平板法分离鼠疫噬菌体,...

Isolation and identification of plague phage from plague foci in Ancient District,Lijiang, Yunnan

Objective To investigate whether the host animals carry plague phage of Ancient District in Lijiang, and we explore the epidemiological significance, to provide scientific basis for prevention and control of host animals in plague foci. Methods Rodent specimens from 13 natural villages of 5 townships in the plague foci in Ancient District of Lijiang District were respectively collected, by 5 m clamp method in September 2017. Vaccine strain EV76 was used as breeding bacteria, phage whose plaque morphology and morphology under electron microscope were identified was isolated from the samples, by double-layer plate method. Results Sampling was carried out in 5 village and towns in the Ancient District of Lijiang, and 766 ileocecum samples were collected from 766 rodents (321 in Qihe , 131 in Wenhua Town, 48 in Kainanliangmei , 117 in Dadong Town, and 149 in Jin'an Town), 34 plague phages were isolated with a total positive rate of 4.43%; Yersinia pestis bacteriophages were isolated from all five foci, of which the positive rate of Qihe (6.85%) was the highest. Of the 34 strains , 29 strains were isolated from Apodemus chevrieri, 2 from Eothenomys miletus and 3 from Crocidura attenuata, Rattus sladerni Andenson and Tupaia belangeri respectively. The positive rate of Apodemus chevrieri was higher than others; There were four kinds of plaque morphology: Outsize (2.5-<3.5 mm), large (1.5-<2.5 mm in diameter), medium (0.5-<1.5 mm) and small (<0.5 mm) in plague phage. Conclusion A certain number of plague phages are carried in the host animals of the plague foci in Gucheng District of Lijiang City, and the phage carrying rate of the Apodemus chevrieri is much higher than that of other species, which is the evidence that Apodemus chevrieri is the principal host in the foci. The polymorphism and microecological relationship with Yersinia pestis need to be analyzed in biology and genomics.