我国东部沿海地区江河入海口水体中致病性弧菌分布特征

目的 分析我国东部沿海江河入海口水域的弧菌分布以及不同季节弧菌种类的差异,为致病性弧菌环境检测策略的制定提供数据支持.方法 分别在2019年8月和2020年1月,在我国濒临渤海、黄海、东海、南海的4个监测地市分别挑选2条河流,在入海口采集水样,进行弧菌分离培养,每份水样挑选30个疑似弧菌菌落,通过微生物质谱仪进行鉴定....

Distribution characteristics of pathogenic Vibrio in estuary of eastern coastal rivers in China

Objective To analyze the distribution of Vibrio in China's coastal rivers and the differences of Vibrio species in different seasons, so as to provide data support for the formulation of environmental detection strategy of pathogenic Vibrio. Methods In August 2019 and January 2020, 2 rivers were selected from 4 monitored cities in Bohai Sea, Yellow Sea, East China Sea and South China Sea, respectively. Water samples were collected at the estuary for isolation and culture of Vibrio. 30 suspected Vibrio colonies were selected from each water sample and identified by microbial mass spectrometer. The distribution characteristics of Vibrio in different sea areas and different seasons were analyzed. Results In Yantai city of Shandong province, Cixi city of Zhejiang province, Cangzhou city of Hebei province, and Fangchenggang city of Guangxi autonomous region, the 32 water samples collected from the four representative cities, 960 strains got from the separation and purification, by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) technical appraisal, in the end, 562 strains of Vibrio were identified, including 211 strains of Vibrio parahaemolyticus (22.0%), 172 strains of Vibrio cholerae (17.9%), 124 strains of Vibrio alginolyticus (12.9%), and 55 strains (5.7%) of the other 8 species. There were significant differences in the distribution of dominant Vibrio in the four sea areas. Vibrio cholerae was dominant in the Yellow Sea (Yantai, Shandong) and the East China Sea (Cixi, Zhejiang), Vibrio parahaemolyticus and Vibrio alginolyticus were dominant in the Bohai Sea (Cangzhou, Hebei), and Vibrio parahaemolyticus was dominant in the South China Sea (Fangchenggang, Guangxi). The dominant strain of Vibrio isolated in summer was Vibrio cholerae (148 strains), accounting for 30.8% of all Vibrio isolated in this season. The dominant strain of Vibrioisolated in winter was Vibrio alginolyticus, a total of 65 strains, accounting for 13.5% of the total Vibrio isolated in the season. There was significant difference in the distribution of dominant Vibrio isolated in summer and winter. Conclusion The distribution of Vibrio in estuaries of rivers in the eastern coastal areas of China is different in sea areas and seasons.