基于网络药理学探讨穿心莲抗癌的作用机制

目的]基于网络药理学探讨穿心莲抗癌的潜在作用机制.方法]应用中药系统药理学技术平台(TCMSP)筛选穿心莲的潜在活性化学物质,借助GeneCards数据库、OMIM数据库平台筛选治疗癌症的作用靶点,利用Cytoscape 3.7.2软件构建"药物-活性化学物质-疾病-作用靶点"网络图,借助String数据库平台构建...

Study on Anti-Cancer Mechanisms of Herba Andrographis Based on Network Pharmacology

Objective To explore the potential mechanisms of Herba Andrographis for anti-cancer effects based on network pharmacology.Methods Traditional Chinese Medicine System Pharmacology Technology Platform(TCMSP)was used to screen out the potential active chemical ingredients from Herba Andrographis.With the help of GeneCards and OMIM database platform,the treatment targets for cancer were selected out.And then a“drugactive chemical ingredient-disease-action target”network diagram was established by Cytoscape 3.7.2 software,and a protein-protein interaction(PPI)network was constructed by String database platform.Finally,the Bioconductor platform and R language were used for gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis.Results A total of 24 potential active ingredients from Herba Andrographis were obtained after screening,involving 71 disease targets,of which 31 were related with cancer.The main active ingredients were wogoin,mono-O-methylwightin,quercetin tetramethyl(3’,4’,5,7)ether,oroxylin A,et al.It mainly exerted antitumor effects by regulating target genes such as AR,ERS1 and IL-6,as well as p53 signaling pathway,thyroid hormone signaling pathway and prolactin signaling pathway,and so on.The main cancer diseases were breast cancer and prostate cancer.Conclusion Network pharmacology is effective for predicting the potential mechanisms for Herba Andrographis against cancer,and the results provide new thoughts and clues for next study.