反向高效液相色谱法同时测定千斤拔不同种、不同药用部位中醋酸乙酯部位化学成分的含量

【目的】同时测定千斤拔的不同种、不同药用部位中醋酸乙酯部位化学成分的含量,以期评价不同种药材的质量,为寻找新药源和新的药用部位提供一定的实验数据。【方法】采用反向高效液相色谱法(RP-HPLC)进行测定。应用Kromasil C18(250 mm×4.6 mm,5μm)色谱柱,流动相为甲醇-0.5%磷酸水(23∶77,10 min;43∶57,11~55 min),流速为1 mL/min,柱温30℃,检测波长为240 nm。【结果】千斤拔燃料木素在2.01504~10.0752μg内呈良好的线性关系,回收率为99.75%,相对标准偏差(RSD)为0.92%;5,7,2’,4’-四羟基异黄酮在2.01042~10.0521μg内呈良好的线性关系,回收率为101.24%,RSD为1.13%;3,5,7,3’,5’-五羟基-4’-甲氧基黄烷在2.01246~10.0623μg内呈良好的线性关系,回收率为102.35%,RSD为1.48%。蔓性千斤拔根、茎中燃料木素和5,7,2’,4’-四羟基异黄酮含量最高,宽叶千斤拔的叶中富含3,5,7,3’,5’-五羟基-4’-甲氧基黄烷,球穗千斤拔仅根、茎含有较少的燃料木素。蔓性千斤拔茎、叶中有效成分的含量远高于其他千斤拔茎、叶的含量。【结论】检测该方法简便、快速、准确。从有效化学成分角度考虑,建议球穗千斤拔不宜替代药典品种蔓性千斤拔、大叶千斤拔入药;从有效化学成分资源的综合及可持续利用角度考虑,建议蔓性千斤拔的茎、叶可以与其根同等入药。

Simultaneous Determination of Contents of Acetic Ether-Extracted Chemical Constituents in Different Medical Parts of Different Species of Radix Flemingiae Philippinensis by RP-HPLC

Objective To simultaneously determine the acetic ether-extracted chemical constituents in different medical parts of different species of Radix Flemingiae Philippinensis,and to evaluate the medicinal material quality of the different species so as to provide references for seeking new medical resource and new medicinal parts.Methods Reversed-phase high-performance liquid chromatographic(RP-HPLC)method was performed on Kromasil C18(250 mm×4.6 mm,5μm)chromatic column with methanol-0.5%phosphoric acid water(23∶77,10 min;43∶57,11-55 min)as mobile phase,flow rate was 1.0 mL/min,column temperature was 30℃,and detection wavelength was 240 nm.Results Genistein displayed good linearity in the range of 2.01504-10.07520μg,average recovery was 99.75%,and relative standard deviation(RSD)was 0.92%.2’-Hydroxygenistein displayed good linearity in the range of 2.01042-10.05210μg,average recovery was 101.24%,and RSD was 1.13%.Ourateacatechin displayed good linearity in therange of 2.01246-10.06230μg,average recovery was 102.35%,and RSD was 1.48%.The contents of genistein and 2’-hydroxygenistein in Moghania philippinensis(Merr.et Rolfe)Li roots and stems was highest,Ourateacatechin was rich in the leaves of ourateacatechin,and little genistein was only in Moghania strobilifera(Linn.)Ait roots and stems.The effective ingredients in stems and leaves of Moghania philippinensis(Merr.et Rolfe)Li were higher than those of other species.Conclusion The established method is simple,accurate,replicate and suitable.From the perspective of effective ingredients,it is suggested that Moghania philippinensis(Merr.et Rolfe)Li and Moghania macrophylla(Willd.)O.Kuntze recorded in pharmacopeia are not replaced with Moghania strobilifera(Linn.)Ait..From the point of comprehensive and sustainable application of effective ingredients resource,it is also suggested that the stems,leaves and roots of Moghania philippinensis(Merr.et Rolfe)Li need to be used as medicine.