不同产地阳春砂内生真菌的分子鉴定研究

[目的]观察广东阳春砂与云南阳春砂内生真菌的种类,比较两地阳春砂内生真菌组成的差异.[方法]采用PDA培养基分离培养广东阳春砂与云南阳春砂茎叶内生真菌,分析两地阳春砂内生真菌的定殖率、相对分离频率.对所获菌株,通过形态特征鉴定归类后,提取菌株的DNA并测序,鉴定菌株的分类地位.[结果]广东阳春砂内生真菌定殖率:茎26....

Study on Molecular Identification of Endophytic Fungi in Amomum villosum Lour.from Different Habitats

Objective To observe the species of endophytic fungi in Guangdong Amomum villosum Lour.and Yunnan Amomum villosum Lour.,and to compare the difference in composition of endophytic fungi in Amomum villosum Lour.from the two habitats of Guangdong and Yunnan.Methods The PDA culture media was used to isolate and cultivate endophytic fungi from the stems and leaves of Guangdong Amomum villosum Lour.and Yunnan Amomum villosum Lour.,and then the colonization rate and relative separation frequency of endophytic fungi in Guangdong Amomum villosum Lour.and Yunnan Amomum villosum Lour.were analyzed.For the achieved strains,after identification and classification were carried out for morphological characteristics,DNA was extracted and sequenced to confirm the taxonomic status of strains.Results The colonization rates of endophytic fungi in Guangdong Amomum villosum Lour.were 26.67%in stems and 54.44%in leaves,and colonization rates of endophytic fungi in Yunnan Amomum villosum Lour.were 15.56%in stems and 43.33%in leaves,Chi-square test showing that the difference was significant(P<0.05 compared with Guangdong Amomum villosum Lour.).The predominant endophytic fungi in Guangdong Amomum villosum Lour.was Colletotrichum,moreover,Alternaria,Aspergillus,Botrytis,Corynespora,Fusarium,Macrophoma,Trichoderma were also isolated.The predominant endophytic fungi of Yunnan Amomum villosum Lour.was Alternaria,moreover,Trichoderma,Fusarium,Schizothyrium,Glomerella,Colletotrichum were also isolated,of which Colletotrichum,Fusarium,Alternaria and Trichoderma were common strains in Guangdong Amomum villosum Lour.and Yunnan Amomum villosum Lour..Conclusion Guangdong Amomum villosum Lour.and Yunnan Amomum villosum Lour.have more species of endophytic fungi with significantly different composition,and are of different predominant endophytic fungi.