| 乙肝病毒（Adr）全基因组在转基因小鼠体内（HBV）DNA的存在状态 |
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| 引用本文： | 张萍萍,万大方,成国祥,励雁峰,成勇,顾健人. 乙肝病毒（Adr）全基因组在转基因小鼠体内（HBV）DNA的存在状态[J]. 肿瘤, 1995, 0(2) |
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| 作者姓名： | 张萍萍 万大方 成国祥 励雁峰 成勇 顾健人 |
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| 作者单位： | 上海市肿瘤研究所，扬州大学农学院生物技术研究所 |
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| 摘 要： | 本文用ＰＣＲ，ＳｏｕｔｈｅｒｎＢｌｏｔ方法检测了３４只Ｇｏ代２６只转基因小鼠携带ＨＢＶＤＮＡ（阳性率为７６％），５１５只Ｇ１代转基因小鼠中２４８只组织中携带ＨＢＶＤＮＡ（阳性率为４８％），２５只Ｇ２代转基因小鼠中１８只组织中携带ＨＢＶＤＮＡ（阳性率为７２％）．对１０８只尼组织阳性小鼠血清检测，１０２只小鼠血清呈ＨＨＶＤＮＡ阳性。以上结果证明，ＨＢＶＤＮＡ基因已整合小鼠的染色体中。这些转基因小鼠对研究ＨＢＶ病毒的复制与表达，慢性肝炎以及肝癌发生的机理，治疗乙肝药物的筛选等具有十分重要的价值。
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| 关 键 词： | HBVDNA，转基因小鼠 |
STATE OF HEPATITIS B VIRUS IN TRANSGENIC MOUSE |
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| Zhang Ping-ping, Wan Da-fang, Cheng Guo-xiang et al. STATE OF HEPATITIS B VIRUS IN TRANSGENIC MOUSE[J]. Tumor, 1995, 0(2) |
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| Authors: | Zhang Ping-ping Wan Da-fang Cheng Guo-xiang et al |
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| Abstract: | Hepatitis B virus (HBV) has an extremely narrow host range, and infects and replicates only in human and chimpanzee. The present experiment produced transgenic mice by microinjecting a partial tandem dupli-complete HBV genome adr type into fertilized ova of 34 mice,26 off springs (G0) were producers for HBV (76% HBV positive rate). The HBV genomes were transmitted to the next generation (G1) , and these G1 mice also produced HBV (248/515, 48% positive rate), 18/25 inbred G2 tail tissues carried HBV DNA (72% positive rate). Virus genome DNA was presentin sera of 102/108 G2 mice. Transgenic mouse containing HBV sequences is an useful tool for detailed study of replication and expression of HBV and for pathological study of hepatitis and hepato-cellular carcinoma. |
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| Keywords: | HBV DNA Transgenic mouse |
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