白及薄层色谱条件的改进和主要色谱斑点化学成分的鉴定

目的:改进白及药典薄层色谱条件,并对白及薄层色谱中主要斑点化学成分进行分离鉴定。方法:采用色谱法进行化学成分分离,采用波谱法进行结构鉴定。结果:优化的薄层色谱条件为氯仿—甲醇—水(6:0.5:0.05);并分离得到4个主要化合物,分别为2,7-二羟基-4-甲氧基-9,10-二氢菲;4,4′-二甲氧基-9,9′10,10′-四氢-2,2′,7,7′-四羟基-1,1′-联菲;4,4′-二甲氧基-9,10-二氢-2,2′,7,7′-四羟基-1,1′-联菲;1-(4-羟基苄基)-2-甲氧基-4,7-二羟基-9,10-二氢菲。结论:优化了白及薄层色谱条件,并对主要色谱斑点化学成分进行了鉴定。

Improving TCL Method of Bletilla striata. and Identifying Constituents of the Main Spots in TLC

Objective： To improve the pharmacopoeia TCL method of Bletilla striata, and studies on the chemical constituents of the main spots in TLC. Methods： Isolation were carried out by chromatography, and identified by spectral data and compared with literature data. Result： The better method of thin layer chromatography was changed as CHCl3-MeOH-H2O （6： 0. 5：0. 05）,and 4 compounds were isolated and identified as 4-methoxy-9,10-dihyd-rophenontrene-2,7-diol; 4,4＇-dimethoxy-9,9＇,10,10＇-tetrahydro- 1, 1＇-biphenanthrene]-2,2＇, 7,7＇-tetrol; 4,4＇-dimethoxy-9,10-dihydro-1, 1＇-biphenanthrene]- 2,2＇, 7,7＇-tetrol; 1-（4-hydroxybenzyl）-4-methoxy-9,10-dihydrophenontrene-2,7-diol. Conclusion： To improve TCL method of Bletilla striata, and the chemical constituents of the main spots in TLC were identified.