人原发性肝细胞癌及肝硬变内HBV DNA及HBAg的分布及意义

为了探讨ＨＢＶ与肝细胞癌的发病的关系，作者应用原位分子杂交，免疫组化及它们的双标记方法在４４例肝癌，１７例癌旁肝组织和４２例肝硬变的石蜡切片中检测了ＨＢＶ ＤＮＡ，ＨＢｘＡｇ。研究结果显示，ＨＢＶ ＤＮＡ，ＨＢｘＡｇ和ＨＢｃＡｇ在肝癌组织中的率分别为７０．５％，６５．９％和２０．５％，而在癌旁肝组织内检出率分别为７６．５％，７６．５％和２９．４％，４２例肝硬变组织中的检出率分别为８８．１％，...

Distribution and significance of HBV DNA and HBAg in human primary hepatocellular carcinoma and liver cirrhosis

In order to explore the relationship between HBV and hepatocellular carcinoma (HCC), hepatitis B virus DNA (HBV DNA), HBxAg and HBcAg were detected in paraffin-embedded sections of 44 cases of HCC, 17 cases of cancerous hepatic tissue and 42 cases of liver cirrhosis (LC) by in situ hybridization (ISH), immunohistochemical (IHC) assay and its double labelling technique. The results showed that the detactabili-ty rates of HBV DNA, HBxAg and HBcAg in cancerous tissues were 70. 5%, 65. 9% and 20.5%, respectively, and that of HBV DNA, HBxAg and HBcAg around cancerous tissues were 76. 5%, 76. 5% and 29. 4% respectively. Among the 42 cases of LC, the detection rates of HBV DNA, HBxAg and HBcAg were 88. 1% , 69. 0% and 33. 3% respectively. In this study, the detection rates of HBV DNA, HBxAg and HBcAg around cancerous tissues and in LC were higher than that in hepatoma tissues, but the difference was not statistically significant (F>0. 05). The results of this study suggestd that a relationship between pathogenesis of HCC and chronic persistent infection of HBV is very close.