| 培养软骨细胞硫酸软骨素代谢速率的测定 |
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| 引用本文： | 章净霞,戴维丽,白俊海. 培养软骨细胞硫酸软骨素代谢速率的测定[J]. 北京大学学报(医学版), 1989, 0(Z1) |
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| 作者姓名： | 章净霞 戴维丽 白俊海 |
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| 作者单位： | 北京医科大学生物物理教研室，北京医科大学生物物理教研室，北京医科大学生物物理教研室 |
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| 摘 要： | 应用同位素示踪技术结合细胞培养和生化微量定量方法,建立一种测定软骨细胞和基质中硫酸软骨素代谢速率的方法,为研究多种因素对软骨细胞功能彬响提供了一个灵敏有效的指标。本文报道了实验方法及应用实例。
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| 关 键 词： | 酸性糖胺聚糖 硫酸软骨素 掺入率 转换速率 |
METABOLIC RATE MEASURE OF CHONDROITIN SULFATE FROM CULTURE CHONDROCYTE |
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| Affiliation: | Department of biophysics,Beijing Medical University Zhang Jing-xia |
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| Abstract: | This paper report a method for measuring metabolic rate of chondroitin sulfate from chondrocyte and matrix with isotope trace.cell culture and biochemical microquantitative analysis technique. Metabolic rate is expressed by incorporate rate and transfer rate. This method provide an sensitive index for studying the effect of various factor on chondrocyte function. Experimental technique and applied example is reported. |
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| Keywords: | Chorndroitin sulfatc Incorporate rate Transfer rate |
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