| ADM诱导人肝癌细胞株多重抗药性的形成 |
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| 引用本文： | 姜飚,韦长元,唐东平,刘剑仑,李挺,杨南武.ADM诱导人肝癌细胞株多重抗药性的形成[J].广西医科大学学报,2001,18(6):771-773. |
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| 作者姓名： | 姜飚 韦长元 唐东平 刘剑仑 李挺 杨南武 |
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| 作者单位： | 1. 广西医科大学附属肿瘤医院普瘤科,
2. 广西医科大学附属肿瘤医院免疫实验室, |
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| 基金项目： | 广西区科技厅资助项目 (桂科攻 99190 40 ) |
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| 摘 要： | 目的：建立人肝癌耐药细胞模型，研究其多药耐药机制。方法：对人肝癌细胞株BEL－7404采用阿霉素浓度递增法及高浓度间歇诱导法，建立人肝癌耐药细胞BEL－740／ADM，用MTT法检测其检测其耐药性，用免疫细胞化学法检测细胞p-糖蛋白（p-gp)及多药耐药相关蛋白（MRP）的表达，结果：用此方法建立的肝癌耐药细胞模型BEL－7404／ADM经MTT法检测其对阿霉素的IC50较亲本细胞BEL－7404增加100倍，并对多种抗癌药物产生交叉耐受性。细胞p-gp及MRP表达较亲本细胞有显著增加（P＜0．01），p-pg阳性率为68．7％，MRP阳性率为53．5％，结论：BEL－7404／ADM是一个多药耐药模型，其多药耐药性与p-gp、MRP高度表达有关。
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| 关 键 词： | 肝癌 多药耐药 阿霉素 |
| 修稿时间： | 2001年4月20日 |
MULTIDRUG RESISTANCE FORMATION OF HUMAN HEPATOCARCINOMA CELL IN DUCED BY ADRIAMYCIN |
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| Jiang Biao,Wei Changyuan,Tang Dongping,et al.MULTIDRUG RESISTANCE FORMATION OF HUMAN HEPATOCARCINOMA CELL IN DUCED BY ADRIAMYCIN[J].Journal of Guangxi Medical University,2001,18(6):771-773. |
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| Authors: | Jiang Biao Wei Changyuan Tang Dongping |
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| Abstract: | Objective:To establish human multidrug resistant hepatocarcinoma cell model and investigate its multidrug resistance mechanism Methods:Through progressively increased concentration and intermittent exposure to high concentration ADM, human cell line BEL 7404 was induced to form human multidrug resistant hepatocarcinoma cell line BEL 7404/ADM Its sensitivity to multianticancer agents was evaluated by MTT assay The expressions of p gp and MRP were detected by immunohistochemical method Results:The multidrug resistant model to ADM was established in a short time with these methods The IC 50 of BEL 7404/ADM to ADM increases by 100 times compared with that of BEL 7404 and it resists to multianticancer agents The expressions of p gp and MRP of BEL 7404/ADM were significantly different from that of BEL 7404 Conclusions:BEL 7404/ADM is a multidrug resistant model,in which multidrug resistant ability is related to p gp and MRP |
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| Keywords: | hepatocarcinoma cell multidrug resistance adriamycin |
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