Transplantation of neonatal porcine islets and sertoli cells into nonimmunosuppressed nonhuman primates |
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Authors: | Isaac J R Skinner S Elliot R Salto-Tellez M Garkavenko O Khoo A Lee K O Calne R Wang D Z |
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Affiliation: | Department of Surgery, National University of Singapore, Singapore. |
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Abstract: | A Mexican group reported transplantation of cocultured neonatal porcine islets and Sertoli cells resulting in insulin independence in nonimmunosuppressed type 1 diabetes patients. We have transplanted similar islets alone (naked islets) or cocultured islets with sertoli cells (islet/sertoli cells) into an omental site and other locations of seven nondiabetic, nonimmunosuppressed, nonhuman primates. Porcine endogenous retrovirus was not detected in recipient blood 8 weeks after porcine islet grafts, and porcine C-peptide was detected at a very low level in all animals. Histology examination failed to demonstrate obviously recognizable islets, but in the animals transplanted with islet/Sertoli cells at the omentum site, there were some surviving glucagons, pan-cytokeratin, and inhibin stained cells at 8 weeks. |
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