肺移植术后稳定状态受者T淋巴细胞亚群的动态变化分析

目的 分析肺移植术后稳定状态受者1年内T淋巴细胞亚群的动态变化及影响因素.方法 收集行同种异体肺移植手术且术后处于稳定状态的41例受者的临床资料.采用流式细胞术检测受者术前、术后2周及每个月(术后1年内)外周血T淋巴细胞亚群绝对值和比值.分析受者年龄、性别、体质量指数(BMI)、手术方式、原发性移植物功能障碍(PGD)...

Analysis on dynamic changes of T lymphocyte subsets in recipients with stable graft status after lung transplantation

Objective To analyze the dynamic changes and the influencing factors of T lymphocyte subsets in recipients with stable graft status within 1 year after lung transplantation.Methods Clinical data of 41 recipients with stable graft status after allogeneic lung transplantation were analyzed.The absolute value and ratio of T lymphocyte subsets in peripheral blood from recipients were measured by flow cytometry before operation,2 weeks and each month(within 1 year)after operation,respectively.The effects of age,gender,body mass index(BMI),surgical method,incidence of primary graft dysfunction(PGD)after operation,and primary disease upon the absolute values of T lymphocytes were evaluated.Results Within 1 year after lung transplantation,the absolute values of CD3^+,CD3^+CD4^+,CD3^+CD8^+T lymphocytes and CD4^+/CD8^+ratio were changed over time(all P<0.001).Compared with preoperative values,there was no statistical significance in the absolute values of CD3^+and CD3^+CD4^+T lymphocytes at 12 months after operation(P=0.659,0.109),whereas the absolute value of CD3^+CD8^+T lymphocytes was increased(P=0.02)and the CD4^+/CD8^+ratio was decreased(P<0.001).Age,gender,BMI,surgical method and incidence of PGD after operation exerted no significant effect on the dynamic changes of absolute values of CD3^+CD4^+and CD3^+CD8^+T lymphocytes(all P>0.05).Primary disease before lung transplantation exerted no effect on the changes of CD3^+CD4^+T lymphocytes,whereas the postoperative absolute value of CD3^+CD8^+T lymphocytes was higher in recipients with infectious lung diseases(P<0.05).Conclusions The absolute values of CD3^+,CD3^+CD4^+,CD3^+CD8^+T lymphocytes in recipients with stable graft status after lung transplantation are relatively low in the early stage after lung transplantation,then gradually restore,and stabilize at 6 months after operation.Dynamic changes are not associated with age,gender,BMI,surgical method and incidence of PGD after operation of recipients.