乳鼠心肌细胞酶消化培养和贴块培养法的比较

目的探索经济、简单易行的心肌细胞培养方法。方法选用新生乳大鼠心肌细胞进行0．25％胰酶分离培养和贴块培养两种培养方法。结果用0．25％胰酶消化培养可获得大量的单细胞，但是酶对细胞的损伤比较大，酶消化所受的影响因素比较多，此过程获取单细胞所需的时间比较长；贴块培养法简便易行，得到的细胞结构完整、存活率高，且经济实惠、适合长期研究且需细胞量不多的实验。结论酶分离培养和贴块培养各有优劣势，应根据不同的实验目的选用不同的培养方法。

Comparison of Cardiomyocyte Culture Obtained by Enzymatic Digestion and Tissue Pieces from Newborn Rats

Objeetlve To find an economical and simple way to conduct cardiomyocyte culture. Methods Two methods, the 0.25% lysine-enzymatlc digestion and lissue pieces were designed for culturing cardiomyocytes from newborn rats. Results The 0.25% lysine-enzymatic digestion method provided sufficient amount of cells for research, but it took longer time and showed more pronounced damage to the cardiomyocytes. The tissue pieces method was easier to handle with less damage to the ceils, but the obtained amount of cardiomyocytes was limited. Conclusion Both two methods have their own advantages and disadvantages, and should be chosen depending on actual situation and demands.