Primitive Neuroectodermal Tumors: Ultrastructural and Immunohistochemical Studies |
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| Authors: | Wielislaw Papierz Janusz Alwasiak Pawel Kolasa Zbigniew Wegrzyn Krzysztof Zakrzewski Leszek Polis Maria Debiec-Rychter Pawel P. Liberski |
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| Affiliation: | a Laboratory of Neuropathology, Department of Pathological Anatomy, Medical Academy Lodz, Lodz, Polandb Laboratory of Electron Microscopy and Neuropathology, Laboratory of Tumor Biology, and Division of Histopathology of Cancer, Department of Oncology, Medical Academy Lodz, Lodz, Polandc Department of Neurosurgery, Regional Hospital (Copernicus), Lodz, Polandd Department of Pediatric Neurosurgery, Memorial Hospital, Polish Mother Health Center, Lodz, Poland |
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| Abstract: | We report here ultrastructural and immunohistochemical studies of neuroblastic differentiation in the retrospective (n = 17) and prospective (n = 26) series of primitive neuroectodermal tumors (PNETs). By electron microscopy, neuritelike structures containing parallel-oriented microtubules, adhesive plaque junctions, and pleomorphic dense-core vesicles were found in the majority of tumor specimens while synaptic specializations were very rare. By immunohistochemistry, synapto-physin appeared to be the most reliable marker for neuroblastic differentiation present in the majority of tumors, while 200 kDa neurofilament protein was immunovisualized in a lower proportion of tumors. Glial fibrillary acidic protein (GFAP) was expressed in both reactive astrocytes and in a small proportion of otherwise typical neoplastic cells. We conclude that the majority of PNETs revealed diverse differentiation and that electron microscopy is still the most reliable tool for its detection followed by immunohistochemistry for synaptophysin. |
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| Keywords: | medulloblastoma neuroblastoma synaptophysin PNET ultrastructure |
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