两种方法检测降钙素原的正确度性能评价

目的采用免疫荧光层析法与电化学发光法定量检测降钙素原（PCT），对其正确度性能进行评价。方法连续检测10d，每天取不同浓度的6～7份临床血清样本，其中高值18份，中值22份，低值28份，共计68份样本分别用广州万孚生物技术服务有限公司出品的免疫荧光层析法仪器与配套试剂（实验方法）和罗氏公司的电化学发光法仪器与配套试剂（比较方法）定量检测PCT，分析两种方法检测结果的偏倚和相关性，评估两种方法的可比性。结果两种检测方法配对t检验，t=0．479，单侧t0．05（67）=1．668，故P〉0．05；直线回归检测，相关系数0．992，P〈0．05，回归方程：y=0．960X＋0．257，检测结果具较好一致性，PCT医学决定水平0．5ng／mL和2．0ng／mL处的总符合率为97．06％。结论免疫荧光层析法与电化学发光法检测PCT结果具高度相关性和一致性，能够满足临床检测的基本要求。

Immunofluorescence assay and the electrochemical luminescence method accuracy evaluation of procalcitonin drop test

Objective Using immunofluorescence assay and the electrochemical luminescence method for quantitative detection of procalcitonin（PCT）, to evaluate the accuracy of performance. Methods 10 consecutive days,6 to 7 clinical serum samples with different concentration of every day,the high value of 18,median 22,low value of 28, a total of 68 samples were used in Guangzhou Wanfu Biological Technology Service Co immunofluorescence chromatog- raphy instruments and reagents（Experiment method） and electrochemical Roche chemiluminescence instrument and reagents（comparison method） and quantitative detection of PCT, analysis of both bias and correlation detection re- sults,two methods of evaluating comparability. Results Two methods for the detection of paired t test,t=0. 479, to. 0s（67） = 1. 668, P ~ 0. 05 ~ Linear regression analysis, r = 0. 992, ~ 0. 975. The regression equation ： Y = 0. 960X ＋ 0. 257,the detection results have good consistency,PCT medical decision the total coincidence rate of 97.06 % levels of 0.5ng/ml and 2.0ng/ml. Conclusion Compared with experimental method in the detection of PCT with a high de- gree of correlation and consistency,to meet the basic requirements of clinical detection.