| 高糖浓度对人肾小球系膜细胞的作用 |
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| 引用本文： | 邱红渝,屈燧林,刘晓惠,杨立川.高糖浓度对人肾小球系膜细胞的作用[J].重庆医学,1999,28(6):412-413,443. |
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| 作者姓名： | 邱红渝 屈燧林 刘晓惠 杨立川 |
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| 作者单位： | 华西医科大学第一医院肾内科,610041 |
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| 摘 要： | 目的 模拟相血糖控制不佳出现的糖浓度波动，研究波动性细胞外高糖浓度（ＦＥＨＧ）对肾小球系膜细胞（ＭｓＣ）增殖及其纤维连接蛋白合成的影响。方法 利用四甲基偶氮唑蓝（ＭＴＴ）比色法和竞争抑制ＥＬＩＳＡ法动态观察不同葡萄糖浓度及波动性高糖浓度对ＭｓＣ增殖和ＦＮ合成的作用。结果 高糖浓度抑制ＭｓＣ增殖，此抑制作用和糖浓度成正相关，同时刺激ＭｓＣ的ＦＮ合成增加；
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| 关 键 词： | 肾小球系膜细胞 纤维连结蛋白 高糖浓度 糖尿病肾病 |
The Effects of Fluctuating Extracellular High Glucose Concentration on Glomerular Mesangial Cells |
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| Qiu Hongyu,Qu Suilin,Liu Xiaohui,Yang Lichuan.The Effects of Fluctuating Extracellular High Glucose Concentration on Glomerular Mesangial Cells[J].Chongqing Medical Journal,1999,28(6):412-413,443. |
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| Authors: | Qiu Hongyu Qu Suilin Liu Xiaohui Yang Lichuan |
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| Abstract: | Objective: In Order to observe the effects of hyperlgycemia on Mesangial cell(MsC). The effects of fluctuating extracellular high glucose(FEHG) on the proliferation and fibronectin (Fn) synthesis were studied. Methods:The Fn level in supernatant of culturedd MsC and MsC Proliferation were assessed by means of competitive inhibiting ELISA method and MTT colormetric method respectively. Results: High glucose levls inhibited the MsC proliferation and FEHG had the same inhibition effect. the Fn synthesis in FEHG was more higher than that in high glucose level. Conclusion: Fluctuating changes in extracellular glucose concentration may play an important pathological role for FN synthesis of MsC in patients with diabetes mellitus treated inadequatly. |
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| Keywords: | Mesangial cells Hyperglycemia Fibronectin Diabetic nephropathy |
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