双炔失碳酯对离体黄体、蜕膜和滋养层细胞的影响

本文以细胞的形态和存活率为指标，用米非司酮作为阳性对照，观察了双炔失碳酯对无血清培养的大鼠黄体、人蜕膜和滋养层细胞的直接损伤作用；同时进一步观察了双炔失碳酯对大鼠黄体细胞分泌功能的影响。结果表明：（１）双炔失碳酯对大鼠黄体细胞、人蜕膜和滋养层细胞有直接损伤作用，其ＬＤ５０分别为：１４．３４±０．９μｇ／ｍｌ、１７．３３±４．１μｇ／ｍｌ和３４．８７±４．９μｇ／ｍｌ。在非致死剂量（５μｇ／ｍｌ）下双炔失碳酯未能抑制ｈＣＧ和孕烯醇酮促进大鼠黄体细胞孕酮生成作用；但能极显著地抑制Ｆｏｒｓｋｏｌｉｎ的促孕酮分泌作用。本文结果提示：溶黄体作用是双炔失碳酯抗早孕的主要机理，对于蜕膜和滋养层的直接损伤作用也是其抗早孕有效环节之一。

The Effects of Anordrin of Luteal Cells, Decidual Cells and Trophoblast Cells in Vitro-The Research on the Mechanism for Terminating Early Pregnancy of Anordrin

Taking the morphology and the viability of the cells as the index, the direct effects of anordrin on the serumfree primary cultures of rat luteal cells, human decidual cells and trophoblast cells were observed. The effect of anordrin on the secretion of rat luteal cells was observed at the same time. The results indicates that:(1) Anordrin has the damaging effects on rat luteal cells, human decidual cells and trophoblast cells. The LD 50 was 14.34±0.9 μg/ml,17.33±4.1 μg/ml and 34.87±4.9 μg/ml respectively. (2) At unleathal dose (5 μg/ml),the activity that hCG and pregnenolone stimulated progesterone secretion of rat lutein cells were not effected by anordrin while the stimulating activity of forskolin was inhibited remarkably by it. The results suggested that the luteolytic action is the main mechanism of the termination of early pregnancy by anordrin. It seems the direct damaging effects of anordrin to decidua and cytotrophoblasts also play a role in the termination of early pregnancy.