新型纳米级rhBMP-2磷酸钙骨水泥修复股骨假体翻修时骨缺损的组织学研究

目的探讨新型纳米级复合人类基因重组骨形态发生蛋白-2（rhBMP-2）／磷酸钙骨水泥（CPC），合称CCPC，在修复股骨假体翻修时骨缺损的成骨作用。方法选择8只健康成年杂种犬，每只犬双侧股骨通过配对，均进行人工股骨头置换手术，一侧为实验组，即CCPC组；另一侧为对照组，即同种异体颗粒性松质骨组（MCA组）。术中按临床上AAOS分型Ⅱ型Ⅱ度行股骨近端骨缺损造模，然后分别应用CCPC和MCA两种材料填充股骨近端骨缺损区，同时固定股骨假体。术后3、6个月分别取出各组骨缺损区标本进行大体及组织学切片观察，了解骨缺损的修复情况。结果术后3个月CCPC组与MCA组骨缺损区均见有新骨形成，术后6个月两种植入材料基本被吸收替代，骨一材料结合良好，骨缺损修复良好。结论纳米级复合rhBMP-2／CPC的骨缺损修复能力与MCA相似。

Histology study of rhBMP-2/CPC used in repairing the femoral bone defects in femoral revision

Objective To investigate the ability of bone formation of recombinant human bone morphogenetic protein -2/calcium phosphate cement （ rhBMP-2/CPC ） in nonhuman primate femoral bone defects models. Methods 8 adult dogs were selected and the bilateral femurs were matched into two groups, the experimental group （rhBMP-2/CPC group） and the control group （morsellized cancellous allograft group）. Artificial femoral head replacement and bone defects models following of type Ⅱ as AAOS classification in proximal femur were performed in bilateral femurs of 8 dog. The bone defect were filled with rhBMP-2/CPC or morsellized cancellous allograft （ MCA ） respectively. 4 dogs in each time were killed at 3 and 6 months after operation,and the specimens of all 16 femurs were collected for histological examination. Results New bone formation were detected in both group at 3 months postoperative. At 6 months postoperative, most of the two filling material were replaced by new bone. Condusions rhBMP-2/ CPC is as good as MCA in bone formation