| mGluR1在自发性癫痫大鼠海马中的表达 |
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| 引用本文： | 王清华,杨丹,蔡际群,孙凤. mGluR1在自发性癫痫大鼠海马中的表达[J]. 中国药理学通报, 2008, 24(9) |
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| 作者姓名： | 王清华 杨丹 蔡际群 孙凤 |
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| 作者单位： | 中国医科大学药学院药物毒理学教研室,辽宁,沈阳110001 |
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| 基金项目： | 国家自然科学基金，高等学校博士学科点专项科研项目 |
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| 摘 要： | 目的检测自发性癫痫大鼠海马中mGluR1的表达情况。方法RT-PCR技术检测鼠海马中mGluR1基因的表达;利用免疫组化和Western blot方法检测鼠海马中mGluR1总蛋白的表达。结果自发性癫痫大鼠海马中mGluR1基因总水平下降(P<0.05),免疫组化显示海马DG区和CA3区的mGluR1蛋白表达均下降(P<0.05),CA1区无变化(P>0.05),mGluR1阳性的神经细胞其密度较正常鼠增强;Western blot检测mGluR1蛋白总量下降(P<0.05)。结论自发性癫痫大鼠海马中总mGluR1表达下调,单一细胞表达增强。
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| 关 键 词： | mGluR1 RT-PCR 免疫组化 Western blot 自发性癫痫大鼠 |
Expression of mGluR1 in hippocampus of spontaneously epileptic rats |
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| WANG Qing-hua,YANG Dan,CAI Ji-qun,SUN Feng. Expression of mGluR1 in hippocampus of spontaneously epileptic rats[J]. Chinese Pharmacological Bulletin, 2008, 24(9) |
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| Authors: | WANG Qing-hua YANG Dan CAI Ji-qun SUN Feng |
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| Abstract: | Aim To investigate the expression of mGluR1 in hippocampus of spontaneously epileptic rats(SER) by control study.Methods Total RNA was extracted from hippocampus of SER and Wistar rats,and mGluR1 mRNA expression was detected by RT-PCR;the expression of mGluR1 protein was detected by immunohistochemistry and Western blot.Results mGluR1 mRNA of SER was lower than that of Wistar rats in hippocampus(P<0.05).mGluR1 protein in DG and CA3 regions of SER hippocampus by immunohistochemistry was decreased compared with that in Wistar rats(P<0.05),but in CA1 region no significant differences(P>0.05) were found.In positive cells,mGluR1 proteins were enhanced.By Western blot,total mGluR1 protein was down-regulated in SER hippocampus(P<0.05).Conclusion The total expression of mGluR1 was down-regulated in SER hippocampus,and enhanced in single cells. |
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| Keywords: | mGluR1 RT-PCR Western blot |
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