广西壮族人群类风湿关节炎患者HLA-DR53、DR4基因及其与抗-CCP抗体的相关性研究

目的探讨广西壮族类风湿关节炎(RA)患者人类白细胞抗原(HLA)-DR53、DR4基因发生的频率数及其与抗-CCP抗体相关性。方法研究对象为49例RA患者及54例健康体检者,采用PCR-SSP法检测HLA-DR53、DR4基因;ELISA法检测抗-CCP抗体。结果 1DR4基因出现频率在RA患者与健康者相当(P>0.05)。2DR53基因出现频率在RA患者明显高于健康者(P<0.05)。3HLA-DR4基因阳性患者更易出现抗-CCP抗体阳性(P<0.05)。结论 1我区壮族人群DR4与RA的发病无相关。2DR4基因阳性RA患者更易出现抗-CCP抗体阳性。3DR53基因阳性RA者与抗-CCP抗体阳性无相关。

HLA-DR53,DR4 genes and their association with anti-CCP antibody in patients with rheumatoid arthritis in Guangxi Zhuang population

Objective To investigate the frequencies of human leukocyte antigen（HLA）-DR53,DR4 genes and their associations with rheumatoid arthritis（RA）in Guangxi Zhuang population. Methods Forty-nine patients with RA and 54 healthy individuals were enrolled in this study.A PCR-SSP technique was used to determine HLA-DR53,DR4 genes and an ELISA method was used to measure anti-CCP antibody.Results 1Patients with RA had nearly the same frequencies of DR4 genes as the healthy subjects（P〉0.05）.2RA patients had higher frequency of DR53 than healthy subjects（P〈0.05）.3Positive-anti-CCP antibody easily occurred in patients with positive-HLA-DR4genes（P〈0.05）. Conclusion 1The occurrence of RA is not associated with DR4 in Guangxi Zhuang population.2RA patients with positive-HLA-DR4 genes easily carrypositive-anti-CCP antibody.3RA patients with positive-DR53 genes hveno association with positive-anti-CCP antibody.