| IL-10基因内含子区基因多态性研究 |
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| 引用本文： | 黄孔文,;王海彦,;韦叶生,;廖品琥,;熊滨,;李军,;黄冰,;胡东海,;仇晓文.IL-10基因内含子区基因多态性研究[J].右江民族医学院学报,2014(6):814-816. |
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| 作者姓名： | 黄孔文 ;王海彦 ;韦叶生 ;廖品琥 ;熊滨 ;李军 ;黄冰 ;胡东海 ;仇晓文 |
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| 作者单位： | [1]右江民族医学院附属医院重症医学科,广西百色533000; [2]广东省妇幼保健院麻醉科,广东广州511400; [3]右江民族医学院附属医院检验科,广西百色533000; [4]广西医科大学肿瘤医院麻醉科,广西南宁530021; [5]广西壮族自治区人民医院重症医学科,广西南宁530021 |
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| 基金项目： | 广西自然科学基金重点项目（2011GXNSFD018039）; 广西科技攻关项目（桂科攻1140003B-93）; 广西教育厅科研资助项目（201012MS176） |
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| 摘 要： | 目的比较研究白细胞介素10(IL-10)基因内含子区单核苷酸多态性(SNP)基因型及等位基因在不同地区、种族人群之间的分布情况。方法采用单碱基延伸PCR技术和DNA测序方法来检测广西人群IL-10基因内含子区rs3021094T/G多态性,并与人类基因组计划研究的4个人群(欧洲、非洲、中国北京、日本)以及台湾地区人群SNP分型数据进行比较。结果 rs3021094T/G多态性分布的频率在广西人群男性、女性之间差异无统计学意义(P>0.05)。与日本、中国北京、台湾地区人群比较分布差异无统计学意义(P>0.05);而与欧洲、非洲地区人群比较,分布差异有统计学意义(P<0.05)。结论 IL-10基因内含子区基因多态性在不同地区、种族人群之间的分布有差异。
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| 关 键 词： | 白细胞介素10 内含子 多态性 基因型 |
Genetic polymorphism of interleukin 10 gene intron region |
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| Institution: | Huang Kongwen , Wang Haiyan , Wei Yesheng , Liao Pinhu , Xiong Bin , Li Jun , Huang Bin , Hu Donghai , Qiu Xiaowen (1. Department of Intensive Care Unit, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise,533000, Guangxi, China ; 2. Department of Anesthesiology, Guangdong Women and Children Hospital, Guangzhou 511400, Guangdong, China 3. Department of Clinical Laboratory Medicine, Affiliated Hospital of Youjiang Medical University for Nationalities, Baise,533000, Guangxi, China 4. Department of Anesthesiology, Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, Guangxi, China; 5. Department of Intensive Care Unit, the People＇s Hospital of Guangxi Zhuang Autonomous region, Nanning 530021, Guangxi, China) |
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| Abstract: | Objective To investigate the frequencies of genotype and allele distribution of interleukin 10（IL-10）gene intron region single nucleotide polymorphism（SNP）in Guangxi population,and to analyze allelic and genotypic diversity of IL-10 gene among different regions and races. Methods The polymerase chain reaction-single base extension（PCR-SBE）and DNA sequencing were used to analyze the rs3021094T/G polymorphisms at the IL-10 gene intron region in Guangxi population.The diversity of genotypic and allelic rs3021094T/GT polymorphisms at the intron region of IL-10 in Guangxi population were compared with others（Hapmap-CEU,Hapmap-YRI,Hapmap-HCB,Hapmap-JPT）from the Human Genome Project group（Hapmap）data（http：//www.ncbi.nlm.nih.gov/projects/SNP/）as well as Taiwan of China. Results Compared the frequencies of rs3021094T/G polymorphisms between females and males in Guangxi population,there were no significant differences（P〉0.05）.Compared the frequencies of rs3021094T/G polymorphisms of Guangxi population with HapMap-HCB,HapMap-JPT and Taiwan of China,there were no significant differences（P〉0.05）.But compared the frequencies of rs3021094T/G polymorphisms of Guangxi population with HapMap-CEU,HapMap-YRI population,there were significant differences（P〉0.05）.Conclusion The frequencies of polymorphisms distribution of IL-10 gene at intron region were significant differences among different regions and ethnic groups. |
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| Keywords: | interleukin 10 intron polymorphism genotype |
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