人腺病毒介导白细胞介素24对H22肝癌荷瘤小鼠的抑瘤作用及免疫功能影响的研究

目的研究腺病毒介导白细胞介素24（Ad—hIL-24）基因对H22肝癌荷瘤小鼠的抑瘤作用及其对免疫功能的影响。方法取16只BALB／C小鼠，其中4只未致瘤，12只BALB／C小鼠致瘤后随机分为3组：PBS组、5-氟脲嘧啶（5-Fu）组和Ad—hIL-24组。PBS组和Ad—hIL-24组（×10^7pfu）隔日用药，共注射5次；5-Fu组注射5-Fu，20μg／kg，连续注射5d，停药2d后，再连续注射5d。2周后，称质量，眼眶取血，处死后取瘤体、脾脏、胸腺，检测抑瘤率、胸腺指数、脾脏指数、白细胞总数及淋巴细胞、单核细胞、中性粒细胞数。结果Ad-hIL-24组与5-Fu组的抑瘤率分别为45．08％和52．11％，两者抑瘤效果差异无统计学意义（P〉0．05），而与PBS组比较差异均有统计学意义（均P〈0．05）。与5-Fu组相比，Ad—hIL-24组的胸腺指数、脾脏指数、外周血白细胞总数、淋巴细胞及单核细胞数均增加，差异均有统计学意义（均P〈0．05）。与正常未致瘤组比较，Ad—hIL-24组中性粒细胞显著增加，差异有统计学意义（P〈0．05）。结论Ad-hIL-24可以抑制小鼠H22肝癌的生长，并对机体免疫功能无抑制作用。

Study on Tumor-suppression Effect and Immunostimulatory Activity Caused by Ad-hIL-24 in H22 Hepatoma Mice

Objective To study the tumor-suppressive effect and immunostimulatory activity caused by Ad-hIL-24 on H22 hepatoma mice. Methods The subcutaneous tumor models were estab- lished in 12 mouse with H22 hepatoma, and were randomly divided into 3 groups： PBS group, 5-Fu group and Ad-hIL-24 group. Then PBS, 5-Fu （20μg/kg）, Ad-hIL-24 （1×10^7Ypfu） were given, intra-tumor injections respectively. Additional 4 mice were taken as the controls. After 15 days, 16 mice were sacri- ficed and subcutaneous tumors in 12 H22 hepatoma mice were drawn out,thymuses and spleens of all mice were taken out. The weights of tumor were measured. The ratio of tumor-suppressione, the indexes of the thymus and spleen, the numbers of white blood cells, the number of lymphocytes, mononuclear cells and neutrophils were detected. Results Compared with PBS group, the ratio of tumor-suppression of Ad-hIL-24 and 5-Fu was 45.08% and 52.11%, respectively. Compared with 5-Fu group, Ad-hIL-24 enhanced the indexes of thymuses and spleens, the numbers of white blood cells and lymphocytes signif- icantly （P〈0.05）. The numbers of neutrophils infected with Ad-hIL-24 had significantly increased, com- pared with the control group （P〈0.05）. Conclusion Ad-hIL-24 inhibits H22 hepatoma in mice and have no side effect on immune function.